The aim of this proposal is to prepare monoclonal antibodies against the human breast cancer cell protein 24k. The antibodies will be prepared by the methodology of Kohler and Milstein and be used to establish a radioimmunoassay for 24k in breast cancer biopsies. A study will be done to compare estrogen and progesterone receptor with 24k in predicting responsiveness to endocrine therapy of breast cancer. The antibodies will also be used to select subsets of breast cancer tissue culture cells particularly responsive to estradiol.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Research Project (R01)
Project #
5R01CA011378-18
Application #
3163465
Study Section
Pathology B Study Section (PTHB)
Project Start
1977-12-01
Project End
1987-11-30
Budget Start
1986-12-01
Budget End
1987-11-30
Support Year
18
Fiscal Year
1987
Total Cost
Indirect Cost
Name
University of Texas Health Science Center San Antonio
Department
Type
Schools of Medicine
DUNS #
800772162
City
San Antonio
State
TX
Country
United States
Zip Code
78229
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Ciocca, D R; Fuqua, S A; Lock-Lim, S et al. (1992) Response of human breast cancer cells to heat shock and chemotherapeutic drugs. Cancer Res 52:3648-54
McGuire, W L; Tandon, A K; Allred, D C et al. (1992) Treatment decisions in axillary node-negative breast cancer patients. J Natl Cancer Inst Monogr :173-80
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Andersen, J; Skovbon, H; Poulsen, H S (1989) Immunocytochemical determination of the estrogen-regulated protein Mr 24,000 in primary breast cancer and response to endocrine therapy. Eur J Cancer Clin Oncol 25:641-3

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