We have identified two distinct, mutually nonrecognizing adhesion mechanisms--one Ca?2+?-dependent, the other Ca?2+?-independent--on the surfaces of chick embryonic retinal and optic tectal cells. Activity of the CI, but not of the CD, adhesion system corresponds with the dorso-ventral gradient of retinal adhesiveness implicated by others in guiding retinotectal mapping. The possible role of the CD mechanism in a naso-temporal adhesion gradient is currently under investigation. Activity of both retinal adhesion mechanisms is associated with molecules located in the 130-160 k region of Laemmli gels. We are continuing our efforts to prepare monoclonal antibodies against components of each mechanism for use as specific immunological probes in the detailed molecular analysis of these adhesion phenomena. In those analyses, emphasis will be placed initially upon the possible heterogeneity within each adhesion mechanism, upon the nature of the adhesive bonds, and upon the organization of adhesion molecules in the cell membrane. Dual mechanisms functionally compatible with, but physiologically and immunologically distinct from, those of the retina have been described in several other neural (7-day cerebrum, cerebellum and spinal cord) and nonneural (7-day heart and liver, 3 3/4-day limbbud) chick tissues. Efforts will be made to study in detail the structural and functional relationships between these adhesion mechanisms and those of the retina. (A)

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Research Project (R01)
Project #
5R01CA013605-15
Application #
3163805
Study Section
Cellular Biology and Physiology Subcommittee 1 (CBY)
Project Start
1978-07-01
Project End
1989-03-31
Budget Start
1987-08-01
Budget End
1989-03-31
Support Year
15
Fiscal Year
1987
Total Cost
Indirect Cost
Name
Princeton University
Department
Type
Schools of Arts and Sciences
DUNS #
002484665
City
Princeton
State
NJ
Country
United States
Zip Code
08544
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