Abstract

Our objective is to clarify the role of polyoma T antigens in cell transformation. We will isolate mutants affecting the medium T antigen of polyoma. We will construct recombinant DNA's to allow the T antigens to be synthesized in bacteria, or in high amounts in mammalian cells. We will study functions associated witht the T antigens, particularly a protein kinase activity of unusual specificity in polyoma T antigen immunoprecipitates. We will seek to relate T antigen functions to mechanisms of cell growth control by studying the control of transcription in polyoma infected and transformed cells, and early viral functions such as mitogenic activity and events leading to anchorage-independent growth.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Research Project (R01)
Project #
5R01CA013884-13
Application #
3163841
Study Section
Virology Study Section (VR)
Project Start
1975-09-01
Project End
1986-02-28
Budget Start
1985-03-01
Budget End
1986-02-28
Support Year
13
Fiscal Year
1985
Total Cost
Indirect Cost

  Institution

Name
Salk Institute for Biological Studies
Department
Type
DUNS #
005436803
City
La Jolla
State
CA
Country
United States
Zip Code
92037

  Publications

Yanochko, Gina M; Eckhart, Walter (2006) Type I insulin-like growth factor receptor over-expression induces proliferation and anti-apoptotic signaling in a three-dimensional culture model of breast epithelial cells. Breast Cancer Res 8:R18
Arbet-Engels, C; Tartare-Deckert, S; Eckhart, W (1999) C-terminal Src kinase associates with ligand-stimulated insulin-like growth factor-I receptor. J Biol Chem 274:5422-8
Arbet-Engels, C; Janknecht, R; Eckhart, W (1999) Role of focal adhesion kinase in MAP kinase activation by insulin-like growth factor-I or insulin. FEBS Lett 454:252-6
Kanemitsu, M Y; Jiang, W; Eckhart, W (1998) Cdc2-mediated phosphorylation of the gap junction protein, connexin43, during mitosis. Cell Growth Differ 9:13-21
Kanemitsu, M Y; Loo, L W; Simon, S et al. (1997) Tyrosine phosphorylation of connexin 43 by v-Src is mediated by SH2 and SH3 domain interactions. J Biol Chem 272:22824-31
Warn-Cramer, B J; Lampe, P D; Kurata, W E et al. (1996) Characterization of the mitogen-activated protein kinase phosphorylation sites on the connexin-43 gap junction protein. J Biol Chem 271:3779-86
Lau, A F; Kurata, W E; Kanemitsu, M Y et al. (1996) Regulation of connexin43 function by activated tyrosine protein kinases. J Bioenerg Biomembr 28:359-68
Glenn, G M; Eckhart, W (1995) Amino-terminal regions of polyomavirus middle T antigen are required for interactions with protein phosphatase 2A. J Virol 69:3729-36
Rigaudy, P; Simon, S; Hunter, T et al. (1994) Antibodies specific for the neuronal form of the Src protein elicited by an antigenized antibody. DNA Cell Biol 13:585-91
Srinivas, S; Schonthal, A; Eckhart, W (1994) Polyomavirus middle-sized tumor antigen modulates c-Jun phosphorylation and transcriptional activity. Proc Natl Acad Sci U S A 91:10064-8

Showing the most recent 10 out of 29 publications