Abstract

The basic objective of this research is the elucidation of the mechanism of virus induced neoplastic transformation. The system of Rous sarcoma virus, avian leukosis virus, and chicken cells will be used as a model. We wish to understand the basic mechanism for the genetic interaction between cellular and viral genomes which leads to the generation of oncogenic viruses. In order to understand biochemical basis of cell transformation process induced by avian retroviruses, characterization of products of transforming genes of several sarcoma, and acute leukemia viruses will be made. Finally, we wish to understand the basis of control of expression of viral or cellular src genome by various regulatory elements including viral promoter sequences.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Research Project (R01)
Project #
5R01CA014935-13
Application #
3164051
Study Section
Virology Study Section (VR)
Project Start
1976-05-01
Project End
1986-04-30
Budget Start
1985-05-01
Budget End
1986-04-30
Support Year
13
Fiscal Year
1985
Total Cost
Indirect Cost

  Institution

Name
Rockefeller University
Department
Type
Graduate Schools
DUNS #
071037113
City
New York
State
NY
Country
United States
Zip Code
10065

  Publications

Grandori, C; Hanafusa, H (1988) p60c-src is complexed with a cellular protein in subcellular compartments involved in exocytosis. J Cell Biol 107:2125-35
Stoeckle, M Y; Sugano, S; Hampe, A et al. (1988) 78-kilodalton glucose-regulated protein is induced in Rous sarcoma virus-transformed cells independently of glucose deprivation. Mol Cell Biol 8:2675-80
Hamaguchi, M; Grandori, C; Hanafusa, H (1988) Phosphorylation of cellular proteins in Rous sarcoma virus-infected cells: analysis by use of anti-phosphotyrosine antibodies. Mol Cell Biol 8:3035-42
Sudol, M; Alvarez-Buylla, A; Hanafusa, H (1988) Differential developmental expression of cellular yes and cellular src proteins in cerebellum. Oncogene Res 2:345-55
Wang, L H; Hanafusa, H (1988) Avian sarcoma viruses. Virus Res 9:159-203
Kornbluth, S; Sudol, M; Hanafusa, H (1987) Association of the polyomavirus middle-T antigen with c-yes protein. Nature 325:171-3
Kornbluth, S; Jove, R; Hanafusa, H (1987) Characterization of avian and viral p60src proteins expressed in yeast. Proc Natl Acad Sci U S A 84:4455-9
Garber, E A; Mayer, B J; Jove, R et al. (1987) Analysis of p60v-src mutants carrying lesions involved in temperature sensitivity. J Virol 61:354-60
Jove, R; Kornbluth, S; Hanafusa, H (1987) Enzymatically inactive p60c-src mutant with altered ATP-binding site is fully phosphorylated in its carboxy-terminal regulatory region. Cell 50:937-43
Calothy, G; Laugier, D; Cross, F R et al. (1987) The membrane-binding domain and myristylation of p60v-src are not essential for stimulation of cell proliferation. J Virol 61:1678-81

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