Abstract

The influence of a number of chemotherapeutic agents on the development of cutaneous squamous cell carcinomas and melanomas induced in the hairless mice by ultroviolet energy will be investigated. The chemicals to be studied are primarily cancer chemotherapeutic agents which not only have profound effects on cellular kinetics and melanocyte function but also are being utilized for prolonged periods in non-cancerous diseases. They will be administered systemically or topically depending on the methods utilized in human disease. The formation and growth of the cancers will be monitored. In addition, the acute and chronic effects of these chemicals on DNA synthesis, repair of ultraviolet-damaged DNA, and mitoses formation will be evaluated with radioactive tracer and colcemid techniques. Their influence on various phases of melanocyte function in normal, UV-stimulated, chemical carcinogen-stimulated and malignant melanoma tissue in vivo will be examined by microscopic techniques and dopa staining procedures.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Research Project (R01)
Project #
5R01CA015605-11
Application #
3164221
Study Section
Chemical Pathology Study Section (CPA)
Project Start
1977-08-01
Project End
1986-01-31
Budget Start
1985-02-01
Budget End
1986-01-31
Support Year
11
Fiscal Year
1985
Total Cost
Indirect Cost

  Institution

Name
University of California San Francisco
Department
Type
Schools of Medicine
DUNS #
073133571
City
San Francisco
State
CA
Country
United States
Zip Code
94143

  Publications

Epstein, J H; Cleaver, J E (1992) 3-Aminobenzamide can act as a cocarcinogen for ultraviolet light-induced carcinogenesis in mouse skin. Cancer Res 52:4053-4
Mitchell, D L; Jen, J; Cleaver, J E (1992) Sequence specificity of cyclobutane pyrimidine dimers in DNA treated with solar (ultraviolet B) radiation. Nucleic Acids Res 20:225-9
Epstein, J H (1992) Experimental models for primary melanoma. Photodermatol Photoimmunol Photomed 9:91-8
Stefanini, M; Collins, A R; Riboni, R et al. (1991) Novel Chinese hamster ultraviolet-sensitive mutants for excision repair form complementation groups 9 and 10. Cancer Res 51:3965-71
Mitchell, D L; Applegate, L A; Nairn, R S et al. (1990) Photoreactivation of cyclobutane dimers and (6-4) photoproducts in the epidermis of the marsupial, Monodelphis domestica. Photochem Photobiol 51:653-8
Mitchell, D L; Cleaver, J E; Epstein, J H (1990) Repair of pyrimidine(6-4)pyrimidone photoproducts in mouse skin. J Invest Dermatol 95:55-9
Mitchell, D L; Hartman, P S (1990) The regulation of DNA repair during development. Bioessays 12:74-9
Mitchell, D L; Nguyen, T D; Cleaver, J E (1990) Nonrandom induction of pyrimidine-pyrimidone (6-4) photoproducts in ultraviolet-irradiated human chromatin. J Biol Chem 265:5353-6
Alidina, R; Kikuchi, M; Kashima, M et al. (1988) Cysteine protease and its inhibitor in experimentally produced squamous cell carcinomas in hairless mouse skin. Exp Mol Pathol 49:118-27
Epstein, J H (1988) Photocarcinogenesis promotion studies with benzoyl peroxide (BPO) and croton oil. J Invest Dermatol 91:114-6

Showing the most recent 10 out of 11 publications