Regulation of Transcription by DNA-Protein Complexes

Gralla, Jay D.

Abstract

The project involves investigating the role of specific protein-DNA complexes in the regulation of gene expression. The principal focus is on the role of RNA polymerases and accessory protein factors in regulating the initiation of transcription. Particular attention is directed to the manner in which DNA sequence directs and mutation alters, transcription. Model templates include mutant E. coli lac promoters, and the genome of the eukaryotic simian virus 40. Proteins studied include E. coli RNA polymerase and lac repressor, RNA polymerase II from several eukaryotic sources, and chromatin-association proteins.

Funding Agency

• Agency: National Institute of Health (NIH)
• Institute: National Cancer Institute (NCI)
• Type: Research Project (R01)
• Project #: 5R01CA019941-11
• Application #: 3165223
• Study Section: Pathobiochemistry Study Section (PBC)
• Project Start: 1976-06-30
• Project End: 1987-06-30
• Budget Start: 1986-07-01
• Budget End: 1987-06-30
• Support Year: 11
• Fiscal Year: 1986

Institution

• Name: University of California Los Angeles
• Type: Schools of Arts and Sciences
• DUNS #: 119132785
• City: Los Angeles
• State: CA
• Country: United States
• Zip Code: 90095

Publications

Buchanan, R L; Gralla, J D (1987) Factor interactions at simian virus 40 GC-box promoter elements in intact nuclei. Mol Cell Biol 7:1554-8

Gralla, J D; Sasse-Dwight, S; Poljak, L G (1987) Formation of blocking lesions at identical DNA sequences by the nitrosourea and platinum classes of anticancer drugs. Cancer Res 47:5092-6

Borowiec, J A; Gralla, J D (1987) All three elements of the lac ps promoter mediate its transcriptional response to DNA supercoiling. J Mol Biol 195:89-97

Borowiec, J A; Gralla, J D (1986) High-resolution analysis of lac transcription complexes inside cells. Biochemistry 25:5051-7

Carpousis, A J; Gralla, J D (1985) Interaction of RNA polymerase with lacUV5 promoter DNA during mRNA initiation and elongation. Footprinting, methylation, and rifampicin-sensitivity changes accompanying transcription initiation. J Mol Biol 183:165-77

Meiklejohn, A L; Gralla, J D (1985) Entry of RNA polymerase at the lac promoter. Cell 43:769-76

Gralla, J D (1985) Rapid ""footprinting"" on supercoiled DNA. Proc Natl Acad Sci U S A 82:3078-81

Borowiec, J A; Gralla, J D (1985) Supercoiling response of the lac ps promoter in vitro. J Mol Biol 184:587-98