Abstract

The ultimate goal of this project is to improve the result of chemotherapy in acute nonlymphocytic leukemia by the development and appropriate utilization of clinically relevant in vitro leukemic clonogenic assay and chemotherapy sensitivity-testing system for individual patients with acute nonlymphocytic leukemia. The most important specific aim of this project is to utilize the leukemic clonogenic assay, which has been recently developed, in a prospecive randomized fashion as a decision-aiding tool either to/or not to administer a second-line chemotherapy, mitoxantrone, to patients with refractory acute nonlymphocytic leukemia. The benefit obtained by the patients will be assessed by the achievement of complete remission as well as the prolongation of survival. Therefore this will determine/establish whether this in vitro system can be used to improve the treatment of patients with acute nonlymphocytic leukemia. Also efforts will continuously be made to improve the assay itself and chemotherapy-testing method. All the clinical and cell-culture data will be computerized for rapid and accurate storage, retrieval computation and analysis, and also efficient feed-back of cell-culture data to the clinical investigators.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Research Project (R01)
Project #
2R01CA020717-08A2
Application #
3165362
Study Section
Experimental Therapeutics Subcommittee 2 (ET)
Project Start
1977-01-01
Project End
1988-03-31
Budget Start
1985-04-01
Budget End
1986-03-31
Support Year
8
Fiscal Year
1985
Total Cost
Indirect Cost

  Institution

Name
University of Kansas
Department
Type
Schools of Medicine
DUNS #
016060860
City
Kansas City
State
KS
Country
United States
Zip Code
66160

  Publications

Park, Chan H; Kimler, Bruce F; Yi, Seong Yoon et al. (2009) Depletion of L-ascorbic acid alternating with its supplementation in the treatment of patients with acute myeloid leukemia or myelodysplastic syndromes. Eur J Haematol 83:108-18
Park, C H; Kimler, B F (1994) Tumor cell-selective flow cytometric analysis for DNA content and cytokeratin expression of clinical tumor specimens by ""cross-gating"". Anticancer Res 14:29-36
Park, C H; Kimler, B F; Bodensteiner, D et al. (1992) In vitro growth modulation by L-ascorbic acid of colony-forming cells from bone marrow of patients with myelodysplastic syndromes. Cancer Res 52:4458-66
Park, C H; Kimler, B F (1991) Growth modulation of human leukemic, preleukemic, and myeloma progenitor cells by L-ascorbic acid. Am J Clin Nutr 54:1241S-1246S
Park, C H; Lee, S H; Stephens, R L et al. (1988) Flow cytometry DNA analysis on tumor cell subpopulation of human tumor specimens by exclusion of lymphohemopoietic cells. J Histochem Cytochem 36:705-9
Park, C H; Kimler, B F; Smith, T K (1987) Clonogenic assay combined with flow cytometric cell sorting for cell-cycle analysis of human leukemic colony-forming cells. Anticancer Res 7:129-32
Park, C H (1987) Humoral regulation of human leukemic progenitor cell cycle: the case for turning off the cell cycle. J Lab Clin Med 109:571-4
Park, C H; Stephens, R L; Amare, M (1986) Growth promotion of human leukemic colonies in vitro with human malignant effusions. Anticancer Res 6:187-90
Park, C H (1985) Biological nature of the effect of ascorbic acids on the growth of human leukemic cells. Cancer Res 45:3969-73
Park, C H; Kimler, B F; Smith, T K (1985) Comparison of the supravital DNA dyes Hoechst 33342 and DAPI for flow cytometry and clonogenicity studies of human leukemic marrow cells. Exp Hematol 13:1039-43

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