The aims of the studies described in this proposal are twofold: (1) To identify at a molecular level the elements that control the differential degree of expression of the HCMV genome in different abortively infected cells; (2) to clarify some of the processes which lead to the stimulation of cellular macromolecular synthesis after infection with HCMV and identify the virus functions which play a role in these processes. Specifically we will study: I. The transcription of the HCMV genome and the mRNA accumulation in productively and different abortively infected cells. II. The translational controls exerted on HCMV mRNAs in productively and different abortively infected cells. III. The effects of HCMV infection on the transcription of different cellular genes. IV. The identification of the DNA binding proteins specified by HCMV V. The isolation of ts mutants defective in various DNA binding and immediate-early proteins.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Research Project (R01)
Project #
5R01CA020806-09
Application #
3165379
Study Section
Virology Study Section (VR)
Project Start
1978-02-01
Project End
1987-08-31
Budget Start
1986-02-01
Budget End
1987-08-31
Support Year
9
Fiscal Year
1986
Total Cost
Indirect Cost
Name
Vanderbilt University Medical Center
Department
Type
Schools of Medicine
DUNS #
004413456
City
Nashville
State
TN
Country
United States
Zip Code
37203