Abstract

Human adenoviruses induce tumors in rodents in vivo and can also efficiently transform cells in vitro. For unexplained reasons the in vivo tumorigenic potential of different adenovirus serotypes varies over a broad range, from nontumorigenic to highly tumorigenic. The role of specific adenovirus genes in tumorigenicity will be studied by analysis of tumorigenic potential of viable recombinant viruses constructed from highly oncogenic and nonongocenic adenovirus serotypes, cells transformed by such viruses and cells transformed with cloned fragments of adenovirus DNA. The effects of specific gene products of highly oncogenic and nononcogenic adenoviruses expressed in transformed cells on interaction with host defense mechanisms, particularly cellular immunity, both natural and induced, will be analyzed. Attention also will be paid to modulation of phenotype of transformed cells during tumor development. Relationship of tumorigenic potential of transformed cells and specific parameters of their interaction with extracellular matrix will also be studied. The studies will provide clues for elucidating the mechanisms responsible for the great differences in oncogenic potential of different adenovirus serotypes in vivo.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Research Project (R01)
Project #
2R01CA021196-09A1
Application #
3165481
Study Section
Virology Study Section (VR)
Project Start
1977-09-30
Project End
1991-03-31
Budget Start
1986-04-01
Budget End
1987-03-31
Support Year
9
Fiscal Year
1986
Total Cost
Indirect Cost

  Institution

Name
University of Medicine & Dentistry of NJ
Department
Type
Schools of Medicine
DUNS #
622146454
City
Piscataway
State
NJ
Country
United States
Zip Code
08854

  Publications

Kim, H S; Raskova, J; Degiannis, D et al. (1994) Effects of cyclosporine and rapamycin on immunoglobulin production by preactivated human B cells. Clin Exp Immunol 96:508-12
Sawada, Y; Raskova, J; Fujinaga, K et al. (1994) Identification of functional domains of adenovirus tumor-specific transplantation antigen in types 5 and 12 by viable viruses carrying chimeric E1A genes. Int J Cancer 57:598-603
Hornung, N; Raskova, J; Raska Jr, K et al. (1993) Responsiveness of preactivated B cells to IL-2 and IL-6. Effect of cyclosporine and rapamycin. Transplantation 56:985-90
Mikhail, N; Raska Jr, K; Raskova, J et al. (1993) Effect of verapamil on the IL-2 binding to its active receptor and on the release of IL-2 receptor by activated PBMC. Immunopharmacology 25:29-36
Donati, D; Degiannis, D; Raskova, J et al. (1992) Uremic serum effects on peripheral blood mononuclear cell and purified T lymphocyte responses. Kidney Int 42:681-9
Javier, R; Raska Jr, K; Shenk, T (1992) Requirement for the adenovirus type 9 E4 region in production of mammary tumors. Science 257:1267-71
Donati, D; Degiannis, D; Combates, N et al. (1992) Effects of hemodialysis on activation of lymphocytes: analysis by an in vitro dialysis model. J Am Soc Nephrol 2:1490-7
Degiannis, D; Luke-Gustites, D; Mikhail, N et al. (1992) Binding of phycoerythrin-conjugated interleukin-6 to in vitro-activated human peripheral blood mononuclear cells--effect of immunosuppressive agents and of a calcium channel blocker. Transplantation 54:308-12
Hornung, N; Raskova, J; Raska Jr, K et al. (1992) IL-2 responsiveness of lectin-induced lymphoblasts: soluble IL-2 receptor release and differential in vitro effects of immunosuppressants. Int J Immunopharmacol 14:753-60
Donati, D; Degiannis, D; Homer, L et al. (1991) Immune deficiency in uremia: interleukin-2 production and responsiveness and interleukin-2 receptor expression and release. Nephron 58:268-75

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