An in vitro bioassay developed in this laboratory will be applied to study tumor colony formation by cells from primary explants of human tumors. Such clonogenic tumor cells are considered to be functionally related to tumor stem cells in vivo. The assay has proven applicable to supporting growth from a variety of human tumors and been applied to studies of human tumor biology and drug sensitivity. The current research proposal has several goals relevant to understanding biological modulation by normal host effector cells of in vitro tumor colony growth and to assess clonogenic tumor cell sensitivity to biological response modifiers including cytokines, monoclonal antibodies and effector cells and to assess drug sensitivity and resistance in human cancer. Clinical correlations will be sought in prospective trials of standard and new agents to define the relevance of clonogenic assays to cancer therapy and new biological and drug development.

National Institute of Health (NIH)
National Cancer Institute (NCI)
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Experimental Therapeutics Subcommittee 2 (ET)
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University of Arizona
Schools of Medicine
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Alberts, D S; Dorr, R T; Wunz, T P et al. (1991) In vitro cytotoxicity against fresh human tumors and P388 leukemia predicts the differential in vivo activity of a series of anthracene anticancer drugs. Anticancer Drugs 2:69-77
Taylor, C W; Salmon, S E; Satterlee, W G et al. (1990) A phase I and pharmacokinetic study of intravenous vinzolidine. Invest New Drugs 8 Suppl 1:S51-7
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Scuderi, P; Woo, L; Chong, A S et al. (1990) Modification of the clonogenic assay for the detection of lymphokine activated killer cell activity. J Immunol Methods 128:119-26
Raitano, A B; Scuderi, P; Korc, M (1990) Long-term effect of tumor necrosis factor and gamma interferon in human pancreatic carcinoma cells. Int J Pancreatol 6:109-18
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Scuderi, P; Rippe, R A; Raitano, A B et al. (1989) Human sera and culture supernatants from human tumors and diploid fetal fibroblasts suppress tumor necrosis factor secretion in vitro. J Leukoc Biol 46:34-40
Salmon, S E; Liu, R (1989) Effects of granulocyte-macrophage colony-stimulating factor on in vitro growth of human solid tumors. J Clin Oncol 7:1346-50
Salmon, S E; Soehnlen, B; Dalton, W S et al. (1989) Effects of tumor necrosis factor on sensitive and multidrug resistant human leukemia and myeloma cell lines. Blood 74:1723-7

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