Abstract

The roles of retroviral and host genes in the congenital transmission of avian leukosis viruses (ALVs) will be studied to learn more about the interactions of retroviruses with their hosts. Recombinant DNA technology will be used to generate recombinants of RAV-O (a virus that does not undergo congenital transmission) and RAV-1 (a virus that does undergo congenital transmission) to rigorously define the region of the ALV genome that determines ability to undergo congenital transmission. DNAs from oviducts infected with viruses that do and do not undergo congenital transmission will be analyzed to determine whether the step in the retroviral life cycle that restricts congenital transmission occurs before integration of proviral DNA into oviduct DNA. The replication of viruses that do and do not undergo congenital transmission will be examined in various tissues to learn if the """"""""oviduct restriction"""""""" occurs in other tissues. Recombinant DNA technology will be used to construct a vaccine virus. Specifically, sequences encoding the type-specific antigens of a subgroup A ALV will be substituted for the comparable sequences of RAV-O ub RAV-O. Such a virus should be immunizing for subgroup A ALVs while undergoing benign, self-limited infections. The K28 line of chickens will be maintained and further selected for endogenous viruses and susceptibility to endogenous virus infections.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Research Project (R01)
Project #
5R01CA023086-09
Application #
3166014
Study Section
Experimental Virology Study Section (EVR)
Project Start
1977-07-01
Project End
1987-12-31
Budget Start
1986-01-01
Budget End
1986-12-31
Support Year
9
Fiscal Year
1986
Total Cost
Indirect Cost

  Institution

Name
Worcester Foundation for Biomedical Research
Department
Type
DUNS #
City
Shrewsbury
State
MA
Country
United States
Zip Code
01545

  Publications

Justewicz, D M; Morin, M J; Robinson, H L et al. (1995) Antibody-forming cell response to virus challenge in mice immunized with DNA encoding the influenza virus hemagglutinin. J Virol 69:7712-7
Foster, R G; Robinson, H L (1994) Establishment of interference in osteoblasts by an osteopetrosis-inducing avian leukosis virus. Virology 205:376-8
Foster, R G; Lian, J B; Stein, G et al. (1994) Replication of an osteopetrosis-inducing avian leukosis virus in fibroblasts, osteoblasts, and osteopetrotic bone. Virology 205:179-87
Robinson, H L; Hunt, L A; Webster, R G (1993) Protection against a lethal influenza virus challenge by immunization with a haemagglutinin-expressing plasmid DNA. Vaccine 11:957-60
Taglienti-Sian, C A; Banner, B; Davis, R J et al. (1993) Induction of renal adenocarcinoma by a nonmutated erbB oncogene. J Virol 67:1132-6
Fynan, E F; Webster, R G; Fuller, D H et al. (1993) DNA vaccines: protective immunizations by parenteral, mucosal, and gene-gun inoculations. Proc Natl Acad Sci U S A 90:11478-82
Robinson, H L; Ramamoorthy, L; Collart, K et al. (1993) Tissue tropism of avian leukosis viruses: analyses for viral DNA and proteins. Virology 193:443-5
Fynan, E F; Robinson, H L; Webster, R G (1993) Use of DNA encoding influenza hemagglutinin as an avian influenza vaccine. DNA Cell Biol 12:785-9
Brown, D W; Kawaoka, Y; Webster, R G et al. (1992) Assessment of retrovirus-expressed nucleoprotein as a vaccine against lethal influenza virus infections of chickens. Avian Dis 36:515-20
Robinson, H L; Tracy, S E; Nair, N et al. (1992) Characterization of an angiosarcoma-inducing mutation in the erbB oncogene. Oncogene 7:2025-30

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