Large numbers of normal newborn infants are systematically exposed to a new and potentially harmful environmental agent routinely utilized for the treatment of neonatal jaundice - high intensity illumination with visible light or phototherapy - often for extended periods of time. The proposed research project will enable us to further characterize the DNA-modifying activity of phototherapy for eukaryotic cells in culture by analyzing the physical chemical properties of DNA isolated from cells exposed to phototherapy in vitro. We will then determine if similar DNA-modification occurs in animals exposed to various phototherapy regimens and determine the action spectra for these genetic effects in order to facilitate the development of a phototherapy unit effective in the treatment of hyperviliruminemia yet devoid of DNA-modifying activity.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Research Project (R01)
Project #
5R01CA023692-08
Application #
3166206
Study Section
Chemical Pathology Study Section (CPA)
Project Start
1977-09-30
Project End
1986-12-31
Budget Start
1985-01-01
Budget End
1985-12-31
Support Year
8
Fiscal Year
1985
Total Cost
Indirect Cost
Name
Case Western Reserve University
Department
Type
Schools of Medicine
DUNS #
077758407
City
Cleveland
State
OH
Country
United States
Zip Code
44106
Ennever, J F; Costarino, A T; Polin, R A et al. (1987) Rapid clearance of a structural isomer of bilirubin during phototherapy. J Clin Invest 79:1674-8
Malhotra, V; Greenberg, J W; Dunn, L L et al. (1987) Fatty acid enhancement of the quantum yield for the formation of lumirubin from bilirubin bound to human albumin. Pediatr Res 21:530-3
Ennever, J F; Knox, I; Speck, W T (1986) Differences in bilirubin isomer composition in infants treated with green and white light phototherapy. J Pediatr 109:119-22
Ennever, J F (1986) Phototherapy in a new light. Pediatr Clin North Am 33:603-20
Malhotra, V; Ennever, J F (1986) Determination of the relative detector response for unstable bilirubin photoproducts without isolation. J Chromatogr 383:153-7
Ennever, J F; Malhotra, V (1986) Current concepts in phototherapy. Indian J Pediatr 53:599-605
Costarino Jr, A T; Ennever, J F; Baumgart, S et al. (1985) Effect of spectral distribution on isomerization of bilirubin in vivo. J Pediatr 107:125-8
Ennever, J F; Knox, I; Denne, S C et al. (1985) Phototherapy for neonatal jaundice: in vivo clearance of bilirubin photoproducts. Pediatr Res 19:205-8
Knox, I; Ennever, J F; Speck, W T (1985) Urinary excretion of an isomer of bilirubin during phototherapy. Pediatr Res 19:198-201
Costarino, A T; Ennever, J F; Baumgart, S et al. (1985) Bilirubin photoisomerization in premature neonates under low- and high-dose phototherapy. Pediatrics 75:519-22