Abstract

Evidence is mounting that papillomaviruses (HPV) are associated with a number of human cancers. In this proposal, we will experimentally generate HPV-infected/transformed human tissues that are activated at the level of gene expression. Activated or amplified genes will be identified in cDNA libraries constructed from the mRNA pool of the tissues. Genes that will be examined include virus specific and cellular genes. Experimentally infected/transformed tissues will also be compared with normal equivalents for susceptibility to mutagenesis.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Research Project (R01)
Project #
5R01CA025305-10
Application #
3166810
Study Section
Chemical Pathology Study Section (CPA)
Project Start
1979-04-01
Project End
1991-01-31
Budget Start
1989-02-01
Budget End
1990-01-31
Support Year
10
Fiscal Year
1989
Total Cost
Indirect Cost

  Institution

Name
Pennsylvania State University
Department
Type
Schools of Medicine
DUNS #
129348186
City
Hershey
State
PA
Country
United States
Zip Code
17033

  Publications

McGarrity, T J; Peiffer, L P; Neely, E B et al. (1996) Localization of protein kinase C alpha isoform expression in the human gastrointestinal tract. Cell Growth Differ 7:953-9
Marks, G; Choudhury, T; Howett, M K (1990) Phenotypic properties of herpesvirus-transformed cells with high tumorigenic and metastatic ability. Virus Res 15:27-44
Howett, M K; Kreider, J W; Cockley, K D (1990) Human xenografts. A model system for human papillomavirus infection. Intervirology 31:109-15
Burdett, L A; Docherty, J J; Howett, M K (1990) Nucleocapsid, nuclear association, and genome location of the herpes simplex virus type 2 38-kD DNA-binding protein. Intervirology 31:76-84
Choudhury, S; Pegg, A E; Kreider, J W et al. (1989) Administration of ethylnitrosourea to neonate hamsters increases growth and frequency of SV40-induced fibrosarcomas. Proc Soc Exp Biol Med 192:72-7
Dollard, S C; Chow, L T; Kreider, J W et al. (1989) Characterization of an HPV type 11 isolate propagated in human foreskin implants in nude mice. Virology 171:294-7