Abstract

Structure-function analyses of murine Ia molecules are being approached through the in vitro production and characterization of B hybridomas that express mutant Ia molecules. Mutant Ia-bearing cells are being produced by chemical mutagenesis and immunoselection. The resulting mutants are analyzed serologically with a panel of monoclonal anti-Ia antibodies, functionally with a panel of class II-restricted T cells, biochemically by two-dimensional gel and peptide map analyses, and by DNA sequence analysis of the mutated gene. (LB)

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Research Project (R01)
Project #
5R01CA026297-07
Application #
3167240
Study Section
Pathobiochemistry Study Section (PBC)
Project Start
1980-01-01
Project End
1987-12-31
Budget Start
1986-01-01
Budget End
1986-12-31
Support Year
7
Fiscal Year
1986
Total Cost
Indirect Cost

  Institution

Name
Mayo Clinic, Rochester
Department
Type
DUNS #
City
Rochester
State
MN
Country
United States
Zip Code
55905

  Publications

Wade, W F; Khrebtukova, I; Schreiber, K L et al. (1995) Truncated MHC class II cytoplasmic and transmembrane domains: effect on plasma membrane expression. Mol Immunol 32:433-46
Wei, B Y; Schreiber, K; Buerstedde, J M et al. (1994) Substitution of class II alpha chain polymorphic residues defines location of A alpha k serologic epitopes and alters association between alpha beta and Ii polypeptides. Int Immunol 6:297-305
Schreiber, K L; Bell, M P; Huntoon, C J et al. (1994) Class II histocompatibility molecules associate with calnexin during assembly in the endoplasmic reticulum. Int Immunol 6:101-11
Beck, B N; Nilson, A E; Bell, M P et al. (1991) The contribution of beta-strand residues to serologic epitopes on the A beta k polypeptide. Immunogenetics 33:409-12
Wei, B Y; Buerstedde, J M; Bell, M et al. (1991) Functional effects of N-linked oligosaccharides located on the external domain of murine class II molecules. J Immunol 146:2358-66
Lee, J M; McKean, D J; Watts, T H (1991) Functional mapping of MHC class II polymorphic residues. The alpha-chain controls the specificity for binding an Ad-versus an A k-restricted peptide and the beta-chain region 65-67 controls T cell recognition but not peptide binding. J Immunol 146:2952-9
Esch, T R; McKean, D J; Thomas, D W (1990) The role of Ia in the formation of a T cell antigen-recognition complex. J Immunol 145:3979-84
Beck, B N; McKean, D J (1990) Mapping T cell receptor epitopes on the A beta polypeptide. Immunol Res 9:47-56
Rosloniec, E F; Vitez, L J; Beck, B N et al. (1989) I-Ak polymorphisms define a functionally dominant region for the presentation of hen egg lysozyme peptides. J Immunol 143:50-8
Esch, T R; McKean, D J; Thomas, D W (1989) Identification of I-A beta-chain residues critical for T cell recognition of peptide antigens. Cell Immunol 123:226-35

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