The goal is to study human leukemias and non-Hodgkin's lymphomas using a multidisciplinary approach encompassing morphology, immunologic markers, cytogenetics, and flow microfluorometric analysis of DNA content, cell size, and cell kinetics in order to improve characterization and classification of these diseases. Cell suspensions and frozen tissues from more than 750 cases of non-Hodgkin's lymphomas, lymphocytic leukemias, and reactive lymphoproliferative conditions have been studied during the past year. We measured adenosine deaminase and purine nucleoside phosphorylase activities by using a linked enzyme spectrophotometric assay on peripheral blood leukemia cells from seven patients with chronic lymphocytic leukemia, three patients with prolymphocytic leukemia and one patient with prolymphocytoid transformation of chronic lymphocytic leukemia. Our studies suggest that purine nucleoside phosphorylase activity in leukemic cells may be useful in distinguishing prolymphocytic leukemia from chronic lymphocytic leumemia, and that it may be an enzymatic marker for the early detection of prolymphocytoid transformation of chronic lymphocytic leukemia. We have described the presence of monocytoid B lymphocytes (MBL) in 17 of 22 lymph nodes from 20 patients with Acquired Immune Deficiency Syndrome (AIDS) and AIDS-related lymphadenopathy. In all 17 samples, the MBL were found in lymph nodes showing florid reactive follicular hyperplasia. MBL were not detected, however, in lymph nodes showing involuted follicles or lymphocyte depletion. Disappearance of MBL apparently parallels the progressive involution of secondary follicles. The diminished T-helper status in AIDS may be relevant to the pathogenesis of follicular involution and indirectly to the disappearance of MBL. Peripheral T-cell lymphomas (PTCL) have been shown to be a morphologically heterogenous group of non-Hodgkin's lymphomas. We have studied PTCL occurring in a 13-year-old girl from whom we were able to evaluate histologic material from 11 biopsies obtained during her 8-month clincial course. These biopsies demonstrated morphologic progression from atypical immune reactions (AIR) and diffuse mixed cell lymphoma (DM) at the time of clinical diagnosis to pleomorphic large cell lymphoma (LCL) with erythrophagocytic tumor cells at the time of her death. We conclude that morphologic progression in PTCL is expressed, in sequential biopsies, by a progressive cellular pleomorphism with the appearance of large, often bizarre lymphoid cells, and the gradual diminuition and disappearance of the B-cell areas and epitheloid histiocytic areas which were present initially. (4)

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Research Project (R01)
Project #
5R01CA026422-08
Application #
3167319
Study Section
Pathology B Study Section (PTHB)
Project Start
1983-05-01
Project End
1988-03-31
Budget Start
1987-04-01
Budget End
1988-03-31
Support Year
8
Fiscal Year
1987
Total Cost
Indirect Cost
Name
City of Hope National Medical Center
Department
Type
DUNS #
City
Duarte
State
CA
Country
United States
Zip Code
91010
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Stroup, R; Sheibani, K (1992) Antigenic phenotypes of hairy cell leukemia and monocytoid B-cell lymphoma: an immunohistochemical evaluation of 66 cases. Hum Pathol 23:172-7
Stroup, R M; Burke, J S; Sheibani, K et al. (1992) Splenic involvement by aggressive malignant lymphomas of B-cell and T-cell types. A morphologic and immunophenotypic study. Cancer 69:413-20
Shin, S S; Sheibani, K; Kezirian, J et al. (1992) Immunoarchitecture of normal human bone marrow: a study of frozen and fixed tissue sections. Hum Pathol 23:686-94
Shin, S S; Sheibani, K; Fishleder, A et al. (1991) Monocytoid B-cell lymphoma in patients with Sjogren's syndrome: a clinicopathologic study of 13 patients. Hum Pathol 22:422-30
Stroup, R; Sheibani, K; Misset, J L et al. (1990) Surface immunoglobulin-positive lymphoblastic lymphoma. A report of three cases. Cancer 65:2559-63
Sheibani, K; Ben-Ezra, J; Swartz, W G et al. (1990) Monocytoid B-cell lymphoma in a patient with human immunodeficiency virus infection. Demonstration of human immunodeficiency virus sequences in paraffin-embedded lymph node sections by polymerase chain reaction amplification. Arch Pathol Lab Med 114:1264-7
Stroup, R M; Sheibani, K; Moncada, A et al. (1990) Angiotropic (intravascular) large cell lymphoma. A clinicopathologic study of seven cases with unique clinical presentations. Cancer 66:1781-8
Wu, A M; Ben-Ezra, J; Winberg, C et al. (1990) Analysis of antigen receptor gene rearrangements in ethanol and formaldehyde-fixed, paraffin-embedded specimens. Lab Invest 63:107-14
Traweek, S T; Ben-Ezra, J; Braziel, R M et al. (1990) The in-vitro response of CD2-positive acute myelogenous leukemia to proliferation and differentiation inducing agents. Leuk Res 14:433-40

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