Abstract

We have shown that the induction of photoreactivatable damage in E. coli AB2480 by high-energy X-ray is due to the associated Cerenkov emission. By use of various experimental techniques we have been able to maximize the yield of photoreactivatable damage as measured by cell survival with and without photoreactivation. We now propose to measure the yield of thymine dimers in DNA of X-irradiated E. coli AB2480 under similar experimental conditions to our cell survival studies. If thymine dimers are the sole photoreactivatable lesions, then survival data and thymine dimer yield measurements should correlate.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Research Project (R01)
Project #
5R01CA027561-05
Application #
3167689
Study Section
Radiation Study Section (RAD)
Project Start
1980-08-01
Project End
1987-01-31
Budget Start
1985-02-01
Budget End
1986-01-31
Support Year
5
Fiscal Year
1985
Total Cost
Indirect Cost

  Institution

Name
University of California Irvine
Department
Type
Schools of Medicine
DUNS #
161202122
City
Irvine
State
CA
Country
United States
Zip Code
92697

  Publications

Redpath, J L (1986) UV-type damage associated with ionizing radiation: a review. Int J Radiat Biol Relat Stud Phys Chem Med 50:191-203
Redpath, J L; Thaden, J J; Fong, R L (1986) Mutation induction in Escherichia coli WP2 uvrA by Cerenkov emission associated with 137Cs gamma irradiation. Radiat Res 105:8-17