The synthesis and in vitro and in vivo evaluation of a series of folate antagonists are proposed, with the primary goal being to develop therapeutically useful anticancer agents. The compounds to be investigated are analogs of folic acid and the well-known anticancer drug methotrexate. Major emphasis will be placed on development of further analogs of 8,10-dideazafolates and 5,10-dideazafolates, effective agents developed in our laboratories. The analogs are to be evaluated in vitro for their effects against folate-dependent bacteria and L1210 leukemia cells and their associated folate enzymes. Other in vitro work will involve mouse intestine epithelial cells. The results will serve as guidelines for potency, bioavailability, and host-tumor toxicity relationships. The analogs will be assayed in vivo against a variety of transplantable tumors in mice as experimental models. In addition, a detailed study of transport phenomena in normal and tumor cells will be conducted.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Research Project (R01)
Project #
5R01CA028783-09
Application #
3168342
Study Section
Experimental Therapeutics Subcommittee 2 (ET)
Project Start
1980-07-01
Project End
1989-06-30
Budget Start
1988-07-01
Budget End
1989-06-30
Support Year
9
Fiscal Year
1988
Total Cost
Indirect Cost
Name
Sri International
Department
Type
DUNS #
City
Menlo Park
State
CA
Country
United States
Zip Code
94025
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