Abstract

Infection with hepatitis B in man is a major health problem of worldwide concern. Its involvement in chronic liver disease and primary hepatocellular caricinoma among carriers signifies the importance of investigating the basic biology of this virus, which is crucial to the development of safe, economic and practical procedures of control of HBV infection. The general aim of the proposed studies is to continue to understand the genetic structure of the HBV genome, investigate the mechanism of viral pathogenesis and carefully examine the oncogenic role of HBV in hepatocarcinogenesis. The major emphasis will be placed on the development and extensive use of expression vectors programmed to synthesize HBV genes in mammalian cells. Approaches will be designed to determine DNA sequences with regulatory functions, enhancer functions and functions whose expression leads to the malignant transformation of cells in culture.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Research Project (R01)
Project #
5R01CA033135-08
Application #
3171095
Study Section
Pathology B Study Section (PTHB)
Project Start
1982-07-01
Project End
1995-11-30
Budget Start
1991-12-01
Budget End
1992-11-30
Support Year
8
Fiscal Year
1992
Total Cost
Indirect Cost

  Institution

Name
University of Colorado Denver
Department
Type
Schools of Medicine
DUNS #
065391526
City
Aurora
State
CO
Country
United States
Zip Code
80045

  Publications

Schaack, J; Maguire, H F; Siddiqui, A (1996) Hepatitis B virus X protein partially substitutes for E1A transcriptional function during adenovirus infection. Virology 216:425-30
Huan, B; Siddiqui, A (1993) Regulation of hepatitis B virus gene expression. J Hepatol 17 Suppl 3:S20-3
Wang, C; Sarnow, P; Siddiqui, A (1993) Translation of human hepatitis C virus RNA in cultured cells is mediated by an internal ribosome-binding mechanism. J Virol 67:3338-44
Huan, B; Siddiqui, A (1992) Retinoid X receptor RXR alpha binds to and trans-activates the hepatitis B virus enhancer. Proc Natl Acad Sci U S A 89:9059-63
Lopez-Cabrera, M; Letovsky, J; Hu, K Q et al. (1991) Transcriptional factor C/EBP binds to and transactivates the enhancer element II of the hepatitis B virus. Virology 183:825-9
Hu, K Q; Siddiqui, A (1991) Regulation of the hepatitis B virus gene expression by the enhancer element I. Virology 181:721-6
Trujillo, M A; Letovsky, J; Maguire, H F et al. (1991) Functional analysis of a liver-specific enhancer of the hepatitis B virus. Proc Natl Acad Sci U S A 88:3797-801
Maguire, H F; Hoeffler, J P; Siddiqui, A (1991) HBV X protein alters the DNA binding specificity of CREB and ATF-2 by protein-protein interactions. Science 252:842-4
Jameel, S; Siddiqui, A; Maguire, H F et al. (1990) Hepatitis B virus X protein produced in Escherichia coli is biologically functional. J Virol 64:3963-6
Lopez-Cabrera, M; Letovsky, J; Hu, K Q et al. (1990) Multiple liver-specific factors bind to the hepatitis B virus core/pregenomic promoter: trans-activation and repression by CCAAT/enhancer binding protein. Proc Natl Acad Sci U S A 87:5069-73

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