This research has as its goal the study of the mechanism of induction of gamma interferon synthesis by human thymocytes in vitro. It is based on our original observation that human thymocytes can be induced to synthesize interferon by two agents: a lectin and products of B-lymphoblastoid cell lines. This is the first observation of induction of gamma interferon, a lymphokine, by precursors of T lymphocytes. It is our aim to identify the thymocyte subsets that are induced and to correlate the induction of interferon synthesis with the acquisition of other immune functions characteristic of mature T lymphocytes. T lymphocytes play a crucial role in cellular immune defense, and the recruitment of thymocytes in immune defense against pathogens or tumor cells may be of vital importance. In view of the response of thymocytes to stimuli that cause gamma interferon synthesis, we also plan to investigate whether thymocytes acquire other immune functions characteristic of mature T lymphocytes when interferon synthesis is induced. It is our goal to investigate the immunoregulatory functions of gamma interferon, in particular its effect on T-cell-dependent immunoglobulin synthesis by thymocytes. Furthermore, we continue our investigation of the relationship between interleukin-2 and gamma interferon synthesis and the pharmacologic regulation of gamma IFN synthesis. The mechanism of induction of gamma interferon is of crucial importance for the understanding of one of the factors that influence host response to invasion by tumor cells in lines. (IS)

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Research Project (R01)
Project #
5R01CA033653-04
Application #
3171464
Study Section
Experimental Immunology Study Section (EI)
Project Start
1983-08-01
Project End
1988-03-31
Budget Start
1986-08-01
Budget End
1988-03-31
Support Year
4
Fiscal Year
1986
Total Cost
Indirect Cost
Name
New York University
Department
Type
Schools of Medicine
DUNS #
004514360
City
New York
State
NY
Country
United States
Zip Code
10012
Andria, M L; Reem, G H (2007) Prolactin expression is induced in Jurkat T-cells by beta-catenin LEF-1, AP-1 and cAMP. Biochem Biophys Res Commun 354:598-602
Reem, G H; Ray, D W; Davis, J R (1999) The human prolactin gene upstream promoter is regulated in lymphoid cells by activators of T-cells and by cAMP. J Mol Endocrinol 22:285-92
Reem, G H; Shi, Y; el Rouby, S (1993) FK-506 inhibits the IL-2-independent induction of the activation antigen CD 69. Ann N Y Acad Sci 685:52-4
Reem, G H; el Rouby, S; Shi, Y (1993) Study of the immunosuppressive properties of cyclosporine analogues. Ann N Y Acad Sci 685:336-8
Reem, G H; Han, X; Marcelli, A (1992) Regulation of IL-2 beta receptor expression and beta-chain mRNA by human thymocytes. Clin Exp Immunol 87:150-6
Reem, G H (1992) Molecular mode of action of cyclosporin and FK506 in human thymocytes. J Autoimmun 5 Suppl A:159-65
el Rouby, S; Shi, Y; Reem, G H (1992) Comparison of the properties of the CsA analogs monoacetyl CyC (o-acetyl-threonine2 cyclosporin) and methyl-alanyl CsA (N-methyl-L-alanyl6 cyclosporin);monoacetyl cyclosporin is immunosuppressive without binding to cyclophilin. Clin Exp Immunol 89:136-42
Reem, G H; Duggan, A; Schleuning, M (1989) Immunoregulation and production of tumor necrosis factor alpha by human thymocytes. Cancer Res 49:3568-73
Schleuning, M J; Duggan, A; Reem, G H (1989) Inhibition by chlorpromazine of lymphokine-specific mRNA expression in human thymocytes. Eur J Immunol 19:1491-5
Schleuning, M; Duggan, A; Reem, G H (1988) Cyclosporine does not inhibit the early transducing signals generated by the activation of human thymocytes. Transplant Proc 20:63-8

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