It is proposed to critically evaluate histochemical methods for the detection and localization of receptors for both steroid and protein hormones in human prostatic tumour tissue and to assess the data in relation to the patient's clinical response to endocrine therapy. Immunocytochemical techniques will be used to localize endogenous protein hormones and their binding sites. The topography of steroid hormone binding site will be examined using fluorescein-labelled steroids or steroid conjugates in relation to analyses for nuclear and cytosolic androgen receptor levels determined by routine biochemical procedures. Normal rat prostate and the Dunning R3327 rat prostate tumour tissue will also provide an additional means of evaluating these histochemical procedures. Furthermore, the biochemical nature of the binding moieties of the fluorescent-labelled steroids will be studied in depth, as an integral part of the research programme, using subcellular fractionation, ion-exchange and gel exclusion chromatography. The British Prostate Study Group's criteria of response to therapy will be used for the work, together with the UICC (TNM) classification of clinical data.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Research Project (R01)
Project #
5R01CA034100-02
Application #
3171840
Study Section
(SRC)
Project Start
1984-05-01
Project End
1987-04-30
Budget Start
1985-05-01
Budget End
1986-04-30
Support Year
2
Fiscal Year
1985
Total Cost
Indirect Cost
Name
Welsh National School of Medicine
Department
Type
DUNS #
City
Cardiff
State
Country
United Kingdom
Zip Code