The aim of this application is to characterize specific aspects of the biology of basic fibroblast growth factor (bFGF). Basic FGF interacts with heparan sulfate. We have hypothesized that this interaction forms a complex which is the biologically relevant form of bFGF. We hypothesize that when complexed to heparan sulfate, bFGF is resistant to proteolytic and thermal denaturation, has increased solubility and is an functionally active as fee bFGF. Experiments will be performed to test these ideas. Basic FGF binds to alpha 2 macroglobulin (alpha2M). We shall charactrize the nature of this complex. The rate of formation, its biological activities as well as its possible role in the regulation of extracellular bFGF levels. Finally, we whall test the role of bFGF in development. Female guinea pigs will be immunized, bread, and the offspring examined for developmental abnormalities. Passive immunization and monoclonal antibodies may also used in addition to natural immunization.
| Deryugina, Elena I; Zajac, Ewa; Zilberberg, Lior et al. (2018) LTBP3 promotes early metastatic events during cancer cell dissemination. Oncogene 37:1815-1829 |
| Rifkin, Daniel B; Rifkin, William J; Zilberberg, Lior (2018) LTBPs in biology and medicine: LTBP diseases. Matrix Biol 71-72:90-99 |
| Morkmued, Supawich; Hemmerle, Joseph; Mathieu, Eric et al. (2017) Enamel and dental anomalies in latent-transforming growth factor beta-binding protein 3 mutant mice. Eur J Oral Sci 125:8-17 |
| Recouvreux, M Victoria; Camilletti, M Andrea; Rifkin, Daniel B et al. (2016) The pituitary TGF?1 system as a novel target for the treatment of resistant prolactinomas. J Endocrinol 228:R73-83 |
| Robertson, Ian B; Rifkin, Daniel B (2016) Regulation of the Bioavailability of TGF-? and TGF-?-Related Proteins. Cold Spring Harb Perspect Biol 8: |
| Zilberberg, Lior; Phoon, Colin K L; Robertson, Ian et al. (2015) Genetic analysis of the contribution of LTBP-3 to thoracic aneurysm in Marfan syndrome. Proc Natl Acad Sci U S A 112:14012-7 |
| Robertson, Ian B; Horiguchi, Masahito; Zilberberg, Lior et al. (2015) Latent TGF-?-binding proteins. Matrix Biol 47:44-53 |
| Horiguchi, Masahito; Todorovic, Vesna; Hadjiolova, Krassimira et al. (2015) Abrogation of both short and long forms of latent transforming growth factor-? binding protein-1 causes defective cardiovascular development and is perinatally lethal. Matrix Biol 43:61-70 |
| Dabovic, Branka; Robertson, Ian B; Zilberberg, Lior et al. (2015) Function of latent TGF? binding protein 4 and fibulin 5 in elastogenesis and lung development. J Cell Physiol 230:226-36 |
| Rognoni, Emanuel; Widmaier, Moritz; Jakobson, Madis et al. (2014) Kindlin-1 controls Wnt and TGF-? availability to regulate cutaneous stem cell proliferation. Nat Med 20:350-9 |
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