We recently found that the in vitro generation of cytolytic T lymphocytes (CTL) from mouse spleen cells requires added soluble factors under some circumstances. These include the: (1) generation of CTL specific for alloantigens when the antigen is on tumor cells and the responding spleen cells are from nonimmunized mice; (2) generation of CTL specific for syngeneic tumor antigens when the responding spleen cells are from nonimmunized mice; and (3) generation of antiviral CTL from precursors from immunized mice if certain viral peptides are used. We have begun to characterize the needed factors biochemically and have found that the factor needed for the allogeneic response can come from phorbol myristic acetate and Concanavalin A-stimulated ROM4 cells and from nonstimulated WEHI-3, AFSTL1 and AFSTL2 cells. The WEHI-3 factor is interleukin-3 which also functions in assisting the generation of antiviral CTL using peptide antigens. Our major objective is to purify and characterize the new factors from ROM4, AFSTL1, and AFSTL2 cells both biochemically and functionally. (HF)

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Research Project (R01)
Project #
5R01CA035152-02
Application #
3172814
Study Section
Immunobiology Study Section (IMB)
Project Start
1984-04-01
Project End
1987-03-31
Budget Start
1985-04-01
Budget End
1986-03-31
Support Year
2
Fiscal Year
1985
Total Cost
Indirect Cost
Name
University of Minnesota Twin Cities
Department
Type
Schools of Arts and Sciences
DUNS #
168559177
City
Minneapolis
State
MN
Country
United States
Zip Code
55455