The long term goal of our research is to define the functional roles of src and myc gene products in Drosophila development. To this end, we have documented the expression of these genes during oogenesis and early embryonic development. These studies, which are detailed in the progress report have shown that the three major src-related and the myc-related Drosophila sequences are maternally inherited and are functional as mRNA during early development. We will extend our studies during the next year through the application of two types of in situ analysis and conventional biochemical techniques. src expression during embryogenesis and oogenesis: (1) by in situ hybridizations to histological sections, determine the localizations of src transcripts; (2) analyze the patterns of synthesis of src proteins by immunoprecipitation of [?35?S]methionine-labeled proteins; (3) determine the presence of src protein in various stages of development by antibody staining of nitrocellulose replicas of electrophoretically separated proteins; and (4) determine the intracellular location of src proteins by immunostaining of histological sections of different developmental stages. Genetic approaches to Drosophila src function: (1) use a viral src gene linked to a heat shock promoter as dominant mutation whose expression can be controlled. Biochemical approaches to Drosophila src function: (1) immuno-affinity purification of Drosophila src proteins followed by in vitro kinase assays to determine the functional conservation of the Drosophila proteins. (X)

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Research Project (R01)
Project #
5R01CA035911-02
Application #
3173433
Study Section
Molecular Biology Study Section (MBY)
Project Start
1984-08-01
Project End
1987-07-31
Budget Start
1985-08-01
Budget End
1986-07-31
Support Year
2
Fiscal Year
1985
Total Cost
Indirect Cost
Name
Worcester Foundation for Biomedical Research
Department
Type
DUNS #
City
Shrewsbury
State
MA
Country
United States
Zip Code
Theroux, S J; Wadsworth, S C (1992) Protein-tyrosine kinase activity of alternate protein products of the Drosophila Dsrc28C locus. FEBS Lett 311:1-6
Wadsworth, S C; Muckenthaler, F A; Vincent 3rd, W S (1990) Differential expression of alternate forms of a Drosophila src protein during embryonic and larval tissue differentiation. Dev Biol 138:296-312
Wadsworth, S C (1990) Drosophila src family proteins. Comp Biochem Physiol B 97:403-6
Vincent 3rd, W S; Gregory, R J; Wadsworth, S C (1989) Embryonic expression of a Drosophila src gene: alternate forms of the protein are expressed in segmental stripes and in the nervous system. Genes Dev 3:334-47
Wadsworth, S C; Rosenthal, L S; Kammermeyer, K L et al. (1988) Expression of a Drosophila melanogaster acetylcholine receptor-related gene in the central nervous system. Mol Cell Biol 8:778-85
Gregory, R J; Kammermeyer, K L; Vincent 3rd, W S et al. (1987) Primary sequence and developmental expression of a novel Drosophila melanogaster src gene. Mol Cell Biol 7:2119-27