This document describes a proposal to investigate: (1) the development of malignant lymphoma in homosexual men with persistent, generalized lymphadenopathy (PGL) and AIDS-like immune dysfunction (acquired immunodeficiency syndrome); (2) factors responsible for the development of this state; and (3) the development of PGL or AIDS or malignant lymphoma in sexual contacts of these patients. Patients will be examined at 6 month intervals, and have serial lymph node biopsies and blood specimens taken. The lymph nodes will be investigated for morphologic expression, and for quantitative and qualitative changes in lymphocyte populations. In addition, DNA content analysis, oncogene expression, chromosomal karyotypes, and staining for various viral antigens (EB, CMV, HTLV) will be performed on the nodes. Serial evaluation of immunologic function will be performed at 6 monthly intervals, including B and T lymphocyte numbers and subsets, Interleukin I and II levels, natural killer and macrophage cytotoxicity. Serologic evidence of infection to a variety of agents (CMV, EB, HTLV, Hepatitis B, toxoplasmosis) will be studied. In addition, a careful epidemiologic evaluation will be performed. All of these parameters will be evaluated serially, in order to note changes which may be associated with the development of lymphoma. Patients will be compared to friend controls who are sexual contacts and with other homosexual controls by means of serial evaluation at six month intervals, with epidemiologic questionnaire, viral serologic and immunologic studies, identical to those performed on the patients, in order to discover those factors responsible for the development of the lymphadenopathy syndrome, or for lymphoma, or for AIDS in the normal homosexual population.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Research Project (R01)
Project #
5R01CA036301-02
Application #
3173843
Study Section
Epidemiology and Disease Control Subcommittee 3 (EDC)
Project Start
1984-07-15
Project End
1987-06-30
Budget Start
1985-07-01
Budget End
1986-06-30
Support Year
2
Fiscal Year
1985
Total Cost
Indirect Cost
Name
University of Southern California
Department
Type
Schools of Medicine
DUNS #
041544081
City
Los Angeles
State
CA
Country
United States
Zip Code
90033
Formenti, S C; Turner, R R; de Martini, R M et al. (1989) Immunophenotypic analysis of peripheral blood leukocytes at different stages of HIV infection. An analysis of asymptomatic, ARC, and AIDS populations. Am J Clin Pathol 92:300-7
Freeman, W R; Chen, A; Henderly, D E et al. (1989) Prevalence and significance of acquired immunodeficiency syndrome-related retinal microvasculopathy. Am J Ophthalmol 107:229-35
de Martini, R M; Turner, R R; Formenti, S C et al. (1988) Peripheral blood mononuclear cell abnormalities and their relationship to clinical course in homosexual men with HIV infection. Clin Immunol Immunopathol 46:258-71
Turner, R R; Levine, A M; Gill, P S et al. (1987) Progressive histopathologic abnormalities in the persistent generalized lymphadenopathy syndrome. Am J Surg Pathol 11:625-32
Turner, R R; Boone, D C; Levine, A M et al. (1987) Flow cytometric lymphocyte immunophenotyping in homosexual men with the persistent generalized lymphadenopathy syndrome: a longitudinal study of lymph nodes and blood. Diagn Clin Immunol 5:194-200
Turner, R R; Meyer, P R; Taylor, C R et al. (1987) Immunohistology of persistent generalized lymphadenopathy. Evidence for progressive lymph node abnormalities in some patients. Am J Clin Pathol 88:10-9
Burkes, R L; Abo, W; Levine, A M et al. (1987) Characterization of immunologic function in homosexual men with persistent, generalized lymphadenopathy and acquired immune deficiency syndrome. Cancer 59:731-8
Levine, A M; Gill, P S; Rasheed, S (1986) Human retrovirus-associated lymphoproliferative disorders in homosexual men. Prog Allergy 37:244-58
Levine, A M; Meyer, P R; Gill, P S et al. (1986) Results of initial lymph node biopsy in homosexual men with generalized lymphadenopathy. J Clin Oncol 4:165-9
Burkes, R L; Meyer, P R; Gill, P S et al. (1986) Rectal lymphoma in homosexual men. Arch Intern Med 146:913-5

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