Using whole intact DNA and cloned restriction fragments of DNA prepared from the B95-8 strain of Epstein-Barr virus, we will determine the regions of the virus genome which code for a variety of EBV-specific markers, including the early antigen (EA) complex, virus capsid antigen (VCA), nuclear antigen (EBNA), and membrane antigen (MA). We will also attempt to identify the transforming gene(s) of the B95-8 genome and transform B-lymphocytes and epithelial cells with B95-8 DNA. Transformed cell lines will be characterized for the expression of EBV specific markers. The EBV DNA in those cell lines will also be examined.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Research Project (R01)
Project #
5R01CA036357-03
Application #
3173911
Study Section
Virology Study Section (VR)
Project Start
1984-07-01
Project End
1987-06-30
Budget Start
1986-07-01
Budget End
1987-06-30
Support Year
3
Fiscal Year
1986
Total Cost
Indirect Cost
Name
Ohio State University
Department
Type
Schools of Medicine
DUNS #
098987217
City
Columbus
State
OH
Country
United States
Zip Code
43210