An assay has been developed in which the DNA repair deficiency of the human genetic disease Xeroderma Pigmentosum is transiently complemented by the introduction by microinjection of cytoplasmic poly(A)+RNA derived from Hela cells. This assay will be utilized to isolate a cDNA copy of repair mRNAs by zoom cloning techniques. The cDNA copy will be used as a probe to identify a clone containing the genomic copy of the repair gene from a human, recombinant DNA library. Structural analyses of the cloned genes and biochemical characterization of their expression products will be performed. These studies should help to elucidate the molecular mechanisms of human, DNA excision repair and the pathobiology of XP.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Research Project (R01)
Project #
5R01CA036486-03
Application #
3174086
Study Section
Molecular Biology Study Section (MBY)
Project Start
1984-04-01
Project End
1988-03-31
Budget Start
1986-04-01
Budget End
1987-03-31
Support Year
3
Fiscal Year
1986
Total Cost
Indirect Cost
Name
University of Texas MD Anderson Cancer Center
Department
Type
Hospitals
DUNS #
001910777
City
Houston
State
TX
Country
United States
Zip Code
77030
Legerski, R J; Penkala, J E; Peterson, C A et al. (1990) Removal of 2',3'-dideoxynucleotide residues from injected DNA in Xenopus laevis oocytes. Mutat Res 236:1-7
Legerski, R J; Penkala, J E; Peterson, C A et al. (1987) Repair of UV-induced lesions in Xenopus laevis oocytes. Mol Cell Biol 7:4317-23