For the last several years our laboratory has been concerned with the biochemical genetics of cell surface antigens and with the immunogenetics of bone marrow engraftment. In this proposal, I outline planned research: 1) to analyze the genetics of bone marrow transplantation in mice. 2) to carry out studies on the genetics of beta 2 microglobulin in man by utilizing a restriction- fragment polymorphism of the B2M gene 3) to produce additional Qa/TL monoclonal antibodies 4) to use biochemical methods to study the array of B2M-associated molecules and 5) to analyze the expression of transfected Qa/T1a region genes.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Research Project (R01)
Project #
5R01CA037374-10
Application #
3175233
Study Section
Allergy and Immunology Study Section (ALY)
Project Start
1988-11-15
Project End
1993-05-31
Budget Start
1991-06-01
Budget End
1993-05-31
Support Year
10
Fiscal Year
1991
Total Cost
Indirect Cost
Name
Massachusetts General Hospital
Department
Type
DUNS #
City
Boston
State
MA
Country
United States
Zip Code
02199
Michaelson, J (1993) Cellular selection in the genesis of multicellular organization. Lab Invest 69:136-51
Salant, E; Michaelson, J (1990) TL.8, a new antigenic determinant of the TL system. Immunogenetics 31:410-1
Whitehead, A S; Rits, M; Michaelson, J (1988) Molecular genetics of mouse serum amyloid P component (SAP): cloning and gene mapping. Immunogenetics 28:388-90
Perkins, D L; Michaelson, J; Marshak-Rothstein, A (1987) The lpr gene is associated with resistance to engraftment by lymphoid but not erythroid stem cells from normal mice. J Immunol 138:466-9
Michaelson, J; Boyse, E A; Ciccia, L et al. (1986) Biochemical genetics of TL antigens. Immunogenetics 24:103-14