Structure-Function of Interferon-Gamma and Its Receptors

Yip, Yum K.

Abstract

Various aspects of the actions of human interferon (IFN)-gamma will be examined with the aid of biological and biochemical techniques. Both highly purified natural and recombinant, E. coli-derived IFN-gamma will be employed in these studies. Studies will include evaluations of the relative efficiency of IFN-gamma in inhibiting various viral functions in different cell systems and in some immunoregulatory activities. We shall also continue to explore regulatory interactions between IFN-gamma and some other lymphokines and monokines. Monoclonal antibodies specific for IFN-gamma will be prepared and characterized with respect to their ability to bind to and neutralize natural and recombinant IFN-gamma. (HF)

Funding Agency

• Agency: National Institute of Health (NIH)
• Institute: National Cancer Institute (NCI)
• Type: Research Project (R01)
• Project #: 5R01CA037385-03
• Application #: 3175247
• Study Section: Physiological Chemistry Study Section (PC)
• Project Start: 1984-02-01
• Project End: 1988-01-31
• Budget Start: 1986-02-01
• Budget End: 1987-01-31
• Support Year: 3
• Fiscal Year: 1986

Institution

• Name: New York University
• Type: Schools of Medicine
• DUNS #: 004514360
• City: New York
• State: NY
• Country: United States
• Zip Code: 10012

Publications

Yee, A M; Yip, Y K; Fischer, H D et al. (1990) Serum activity that confers acid lability to alpha-interferon in systemic lupus erythematosus: its association with disease activity and its independence from circulating alpha-interferon. Arthritis Rheum 33:563-8

Yee, A M; Buyon, J P; Yip, Y K (1989) Interferon alpha associated with systemic lupus erythematosus is not intrinsically acid labile. J Exp Med 169:987-93

Zhang, Y H; Lin, J X; Yip, Y K et al. (1988) Enhancement of cAMP levels and of protein kinase activity by tumor necrosis factor and interleukin 1 in human fibroblasts: role in the induction of interleukin 6. Proc Natl Acad Sci U S A 85:6802-5

Bernheimer, A W; Robinson, W G; Linder, R et al. (1987) Toxicity of enzymically-oxidized low-density lipoprotein. Biochem Biophys Res Commun 148:260-6

Tsujimoto, M; Yip, Y K; Vilcek, J (1986) Interferon-gamma enhances expression of cellular receptors for tumor necrosis factor. J Immunol 136:2441-4

Tsujimoto, M; Yip, Y K; Vilcek, J (1985) Tumor necrosis factor: specific binding and internalization in sensitive and resistant cells. Proc Natl Acad Sci U S A 82:7626-30