Preneoplastic steps such as metaplasia, hyperplasia, and atypia are considered pathological steps in the development human carcinoma. Multiphasic oncogene activation during carcinogenesis is shown to occur in malignant neoplasia. Oncogene activation is usually determined by morphological transformation of NIH3T3 cells. It is proposed to demonstrate the activation of oncogenes in tumors induced in human pancreas explants, to determine and compare the temporal relationship between the activation of the oncogene and morphological steps in pancreas carcinogenesis. It is further proposed to develop a system for detection of oncogene activation other than NIH3T3 cells. This model will be based on the morphological transformation of human pancreas cell lines in vitro and their tumorigenicity in """"""""athymic nude"""""""" mice in vivo.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Research Project (R01)
Project #
1R01CA038955-01A1
Application #
3177487
Study Section
Chemical Pathology Study Section (CPA)
Project Start
1985-07-01
Project End
1988-06-30
Budget Start
1985-07-01
Budget End
1986-06-30
Support Year
1
Fiscal Year
1985
Total Cost
Indirect Cost
Name
Suny Downstate Medical Center
Department
Type
Schools of Medicine
DUNS #
068552207
City
Brooklyn
State
NY
Country
United States
Zip Code
11203