Abstract

Monoclonal antibodies to hormone and factor receptors of human cells will be produced. These will be used in various combinations to kill cancer cells in culture and to inhibit tumor growth in nude mice. Screening methods will be developed to search for hybridomas that produce high quantitites of high affinity antibodies to receptors. Screening and selection methods will be developed for searching for mutants which produce high levels of growth factors. Combinations of protein chemistry and recombinant DNA techniques will be used to characterize novel growth factors. (CS)

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Research Project (R01)
Project #
5R01CA040294-05
Application #
3180072
Study Section
Cellular Biology and Physiology Subcommittee 1 (CBY)
Project Start
1985-08-19
Project End
1990-07-31
Budget Start
1989-08-01
Budget End
1990-07-31
Support Year
5
Fiscal Year
1989
Total Cost
Indirect Cost

  Institution

Name
Adirondack Biomedical Research Institute
Department
Type
DUNS #
City
Lake Placid
State
NY
Country
United States
Zip Code
12946

  Publications

Wu, D Q; Kan, M K; Sato, G H et al. (1991) Characterization and molecular cloning of a putative binding protein for heparin-binding growth factors. J Biol Chem 266:16778-85
Myoken, Y; Kayada, Y; Okamoto, T et al. (1991) Vascular endothelial cell growth factor (VEGF) produced by A-431 human epidermoid carcinoma cells and identification of VEGF membrane binding sites. Proc Natl Acad Sci U S A 88:5819-23
Kawamoto, T; Sato, G H; Takahashi, K et al. (1990) Monoclonal antibodies to epidermal growth factor receptors in studies of receptor structure and function. Cytotechnology 3:279-93
Myoken, Y; Kan, M; Sato, G H et al. (1990) Bifunctional effects of transforming growth factor-beta (TGF-beta) on endothelial cell growth correlate with phenotypes of TGF-beta binding sites. Exp Cell Res 191:299-304
Wu, D G; Wang, L H; Chi, Y et al. (1990) Human epidermal growth factor receptor residue covalently cross-linked to epidermal growth factor. Proc Natl Acad Sci U S A 87:3151-5
Wu, D G; Wang, L H; Sato, G H et al. (1989) Human epidermal growth factor (EGF) receptor sequence recognized by EGF competitive monoclonal antibodies. Evidence for the localization of the EGF-binding site. J Biol Chem 264:17469-75
Sato, J D; Cao, H T; Kayada, Y et al. (1988) Effects of proximate cholesterol precursors and steroid hormones on mouse myeloma growth in serum-free medium. In Vitro Cell Dev Biol 24:1223-8
Jacobson, R A; Sato, J D; Sato, G H (1987) Biological screening of monoclonal antibodies. Biochem Biophys Res Commun 149:309-12
Sato, J D; Kawamoto, T; Okamoto, T (1987) Cholesterol requirement of P3-X63-Ag8 and X63-Ag8.653 mouse myeloma cells for growth in vitro. J Exp Med 165:1761-6