Attempts will be made to regulate the expression of the c-src and other cellular proto-oncogenes. The approach will involve construction of plasmids that encode proto-oncogene antisense RNAs that can act to arrest the translation of the homologous mRNA in vivo. Such plasmids will be transfected into polyoma transformed rat and mouse fibroblasts, as well as normal primary and established cells, and teratocarcinoma cells. A long term objective includes the insertion of these plasmids into mouse embryos and establishment of transgenic mice.
| Talmage, D A; Riney, C; Benjamin, T L (1991) Regulation of pp60c-src expression in rat and mouse fibroblasts by an inducible antisense gene: effects on serum regulation of growth and polyoma virus middle T function. Cell Growth Differ 2:51-8 |
