To assess the natural history of Human papillamavirus (HPV) infections in uterine cervix (fat, inverted and papillomatous condylomas) and their associations with cervical intraepithelial neoplasia (CIN) and cervical cancer, a long-term prospective follow-up study was started at our clinic in October 1981. At this writing, a total of 418 women have been followed-up for a mean of 20 15 (M SD) months, either by cervical punch biospy or Papanicolaou (PAP) smears, repeated at 6-month intervals. On each attendance at the Clinic, the patient is subjected to colposcopy. To evaluate the role accompanying cervical infections, cervical swabs (for isolation of Chlamydia trachomatis and HSV), and by serum samples for viral (HPV, HSV, CMV) serology. PAP smears and punch biopsies are analysed for cytopathic changes of HPV (koilocytosis, dyskeratosis), and for concomitant CIN, and the biopsies also for HPV particles on transmission electron microscopy (TEM). Expression of HPV structural proteins is demonstrated with indirect immunoperoxidase (IP-PAP) technique applied on paraffin sections. In fresh biopsy samples (on frozen sections), the immunocampetent cells within the in situ inflammatory infiltrate are phenotypically characterized using monoclonal antibodies (McAB) to define T cell subsets, NK (natural killer) cells and Langerhans cells. Spot and in situ hybridization as well as Sothern blot are used to detect and analyse HPV DNA with cloned HPV type 6, 11, 16 and 18 DNA as probes. Biopsy specimens will also be tested for amplification of the major cellular oncogenes (c-onc). To assess the factors with influence on transmission of cervical HPV infections, all patients are requested for their sexual and smoking habits by a detailed questionnaire. The incidence and prevalence of cervical HPV infections in unselected Finnish female population are determined by an epidemiological study, utilizing the mass-screening programme of the Finnish Cancer Society. The preliminary results suggest that cervical HPV infections seem to be capable of progressing event into CIS, and thus present with the natural history comparable to that of classical CIN.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Research Project (R01)
Project #
1R01CA042010-01
Application #
3182731
Study Section
(SSS)
Project Start
1986-07-01
Project End
1989-06-30
Budget Start
1986-07-01
Budget End
1987-06-30
Support Year
1
Fiscal Year
1986
Total Cost
Indirect Cost
Name
University of Kuopio
Department
Type
DUNS #
368905019
City
Kuopio
State
Country
Finland
Zip Code
Syrjanen, K J (1996) Spontaneous evolution of intraepithelial lesions according to the grade and type of the implicated human papillomavirus (HPV). Eur J Obstet Gynecol Reprod Biol 65:45-53
Hellberg, D; Nilsson, S; Gad, A et al. (1993) Behavior of cervical intraepithelial neoplasia (CIN) associated with various human papillomavirus (HPV) types. Arch Gynecol Obstet 252:119-28
Kataja, V; Syrjanen, S; Yliskoski, M et al. (1993) Risk factors associated with cervical human papillomavirus infections: a case-control study. Am J Epidemiol 138:735-45
Tosi, P; Pallini, V; Cintorino, M et al. (1993) Use of antibodies against a synthetic peptide of the E6 protein of human papillomavirus (HPV) type 16 for the diagnosis of genital HPV lesions. Cytopathology 4:3-15
Chang, F; Syrjanen, S; Kellokoski, J et al. (1991) Human papillomavirus (HPV) infections and their associations with oral disease. J Oral Pathol Med 20:305-17
Syrjanen, S; Saastamoinen, J; Chang, F J et al. (1990) Colposcopy, punch biopsy, in situ DNA hybridization, and the polymerase chain reaction in searching for genital human papillomavirus (HPV) infections in women with normal PAP smears. J Med Virol 31:259-66
Leoncini, P; Petracca, R; Ruggiero, P et al. (1990) Expression of cytokeratin No. 19 polypeptide in genital papillomavirus lesions. Gynecol Obstet Invest 29:59-66
Chang, F; Syrjanen, S; Shen, Q et al. (1990) Detection of human papillomavirus (HPV) in genital warts and carcinomas by DNA in situ hybridization in Chinese patients. Cytopathology 1:97-103
Syrjanen, K; Hakama, M; Saarikoski, S et al. (1990) Prevalence, incidence, and estimated life-time risk of cervical human papillomavirus infections in a nonselected Finnish female population. Sex Transm Dis 17:15-9
Syrjanen, S M (1990) Basic concepts and practical applications of recombinant DNA techniques in detection of human papillomavirus (HPV) infection. Review article. APMIS 98:95-110

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