Although a vast amount of research activities have been directed toward the role of dietary fat in growth of the carcinomatous rodent mammary gland, it is now important to determine whether or not carcinomatous human breast tissues respond to dietary fat as has been amply demonstrated in rodents. Although numerous epidemiological studies in human populations have provided evidence that diets rich in fat may increase the risk for the development of breast cancer, a number of more recent studies have seriously questioned this relationship. Thus, to date, we have only epidemiological data to link dietary fat and human breast cancerigenesis. In this research proposal, we intend to examine this potentially important relationship by determining whether or not dietary fat can affect growth processes of carcinomatous human breast epithelial cells maintained in female athymic nude mice. More specifically, the following four objectives will be pursued. 1.Can the amount and type of dietary fat affect the growth of carcinomatous human breast cells (hormone responsiveness MCF-7, T47-D, ZR75-1 cell lines, hormone nonresponsive Hs578T, MX-1 cell lines)? 2.Is the stimulatory effect of a high fat diet on growth of carcinomatous human breast cells contingent upon an ad libitum type of feeding pattern? 3.Is the inhibitory effect of a fish oil diet on growth of carcinomatous human breast cells a function of increased accumulation of carcinoma lipid peroxides? 4.Does the amount and type of dietary fat effect the growth of carcinomatous human breast cells by altering carcinoma cell proliferation and/or by altering carcinoma cell loss (cytolysis)? In summary, we feel that the research projects outlined in this research proposal are a logical and important extension of our past studies; these studies are not only of conceptual and of mechanistic importance but, in addition, address an important current practical issue.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Research Project (R01)
Project #
5R01CA042876-06
Application #
2090990
Study Section
Chemical Pathology Study Section (CPA)
Project Start
1986-09-01
Project End
1995-06-30
Budget Start
1993-07-01
Budget End
1995-06-30
Support Year
6
Fiscal Year
1993
Total Cost
Indirect Cost
Name
Michigan State University
Department
Pathology
Type
Schools of Osteopathy
DUNS #
193247145
City
East Lansing
State
MI
Country
United States
Zip Code
48824
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Welsch, C W (1994) Interrelationship between dietary lipids and calories and experimental mammary gland tumorigenesis. Cancer 74:1055-62
Welsch, C W; Oakley, C S; Chang, C C et al. (1993) Suppression of growth by dietary fish oil of human breast carcinomas maintained in three different strains of immune-deficient mice. Nutr Cancer 20:119-27
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