The aims of this project are to investigate the natural history of bladder cancer in mice and man using cell and molecular biological approaches. The central approach to this work is the use of gene transfer techniques to study the effects of known transforming genes in different bladder epithelial cell lines, both mouse and human, and to look for alternative routes of transformation in bladder cancer.
The aims of the proposal are: 1. To identify alternative routes of transformation in bladder cancer using NIH/3T3 cells with in vivo selection and by developing new recipients for DNA transfection. 2. To study the expression of characterized transforming genes in different epithelial cell types to establish the effects of the differentiation and growth characteristics of these cells in vitro and in vivo. 3. To examine the timing and expression of ras gene products in bladder carcinogenesis and to study the mode of action of different transforming genes and define cellular targets involved in epithelial transformation.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Research Project (R01)
Project #
5R01CA042944-06
Application #
3184688
Study Section
Pathology B Study Section (PTHB)
Project Start
1986-08-01
Project End
1995-01-31
Budget Start
1992-02-01
Budget End
1993-01-31
Support Year
6
Fiscal Year
1992
Total Cost
Indirect Cost
Name
Beth Israel Deaconess Medical Center
Department
Type
DUNS #
City
Boston
State
MA
Country
United States
Zip Code
02215
Pories, S E; Ramchurren, N; Summerhayes, I et al. (1993) Animal models for colon carcinogenesis. Arch Surg 128:647-53
Weber, T K; Steele, G; Summerhayes, I C (1992) Differential pp60c-src activity in well and poorly differentiated human colon carcinomas and cell lines. J Clin Invest 90:815-21