The long term objective of this proposal is to examine the effect of adoptive T cell transfers in altering immune responsiveness in cancer patients. We are proposing a Phase II clinical trial for the treatment of adenocarcinoma of the lung by intravenous infusion of Interleukin 2-responsive T lymphocytes and systemic Interleukin 2 (IL 2). The lymphocytes to be used in this therapy are tumor infiltrating cells propagated directly from autologous tumor tissue. Two color flow cytometric analyses will be carried out to characterize these lymphocytes and to compare them to lymphocytes derived from blood and pleural fluid from the same patient. The lymphocytes used for therapy will be assessed for functional activity by several criteria including the ability to lyse and respond to autologous tumor when it is available. Patients will receive 1 x 10-10 lymphocytes over a 3 week period during which time they will receive continuous intravenous infustions of 1,000,000 units of recombinant IL 2/M2/24 hours. Patient will be monitored by routine medical and radiographic testing. The IL 2-propagated lymphocytes will be radiolabelled in order to assess the in vivo distribution of these cells. Monitoring of surface phenotypes and functional assessment of changes in peripheral blood lymphocytes will be carried out during therapy. Each patient will receive a second course of therapy following a 30 day interval. Patients will be re-evaluated for evidence of decrease in tumor burden and alterations in immunological parameters.
Showing the most recent 10 out of 12 publications
