Mechanism of Transformation by Bk Virus

Folk, William Robert

Abstract

The long term objective of this research is to elucidate cellular and viral factors which determine the oncogenicity of BK virus. Sequences within the BK virus enhancer will be defined with respect to their importance for gene expression, DNA replication and transformation. Cellular factors which interact with these sequences will be identified, and the mechanism of gene control by the enhancer analyzed. We will determine whether there is a correlation between expression of the early region and development of pathology in transgenic mice. Using cells transformed in culture, and tumor cells obtained from transgenic mice, we will study the mechanisms by which the BK virus tumor antigens induce transformation. We will determine the cellular localization of the BK tumor antigens, and determine whether they associate with known cellular growth regulators at those sites. We will measure the extent of complex formation with p53 and other cellular proteins.

Funding Agency

• Agency: National Institute of Health (NIH)
• Institute: National Cancer Institute (NCI)
• Type: Research Project (R01)
• Project #: 5R01CA045033-05
• Application #: 3188006
• Study Section: Special Emphasis Panel (SRC (47))
• Project Start: 1989-02-01
• Project End: 1994-01-31
• Budget Start: 1992-02-01
• Budget End: 1994-01-31
• Support Year: 5
• Fiscal Year: 1992

Institution

• Name: University of Missouri-Columbia
• Type: Schools of Medicine
• DUNS #: 112205955
• City: Columbia
• State: MO
• Country: United States
• Zip Code: 65211

Publications

Lednicky, J; Folk, W R (1992) Two synthetic Sp1-binding sites functionally substitute for the 21-base-pair repeat region to activate simian virus 40 growth in CV-1 cells. J Virol 66:6379-90

Kang, S; Folk, W R (1992) Lymphotropic papovavirus transforms hamster cells without altering the amount or stability of p53. Virology 191:754-64

Martin, M E; Yang, X Y; Folk, W R (1992) Expression of a 91-kilodalton PEA3-binding protein is down-regulated during differentiation of F9 embryonal carcinoma cells. Mol Cell Biol 12:2213-21

Yoo, W; Martin, M E; Folk, W R (1991) PEA1 and PEA3 enhancer elements are primary components of the polyomavirus late transcription initiator element. J Virol 65:5391-400

Tang, W J; Folk, W R (1989) Constitutive expression of simian virus 40 large T antigen in monkey cells activates their capacity to support polyomavirus replication. J Virol 63:5478-82

Tang, W J; Folk, W R (1989) Asp-286----Asn-286 in polyomavirus large T antigen relaxes the specificity of binding to the polyomavirus origin. J Virol 63:242-9

Sullivan, M; Folk, W R (1988) An improved procedure for measuring DNA replication with transient assays in eukaryotic cells. Gene Anal Tech 5:54-6

Martin, M E; Piette, J; Yaniv, M et al. (1988) Activation of the polyomavirus enhancer by a murine activator protein 1 (AP1) homolog and two contiguous proteins. Proc Natl Acad Sci U S A 85:5839-43