Our long-term objectives are to examine the clinical roles of tuftsin, a naturally-occurring immunomdulating hormone-like peptide, as an antitumor, anti-microbial and immuno-augmenting agent and also to elucidate the mechanism of action of this peptide. Based on our studies the following specific aims are proposed: 1. Examine growth factor activity of tuftsin in vitro. 2. Examine specific binding of tuftsin to various cells using fluorescein-labeled tuftsin and 3H--tuftsin in vitro. 3. Purify and characterize tuftsin receptors (including tuftsin binding). 4. Conduct biochemical study of fate of tuftsin internalized by granulocytes and monocytes. Tuftsin and its anologs will chemically synthesized and purified in this laboratory.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Research Project (R01)
Project #
5R01CA045730-02
Application #
3188962
Study Section
Experimental Therapeutics Subcommittee 2 (ET)
Project Start
1987-07-01
Project End
1990-06-30
Budget Start
1988-07-01
Budget End
1989-06-30
Support Year
2
Fiscal Year
1988
Total Cost
Indirect Cost
Name
University of Texas MD Anderson Cancer Center
Department
Type
Hospitals
DUNS #
001910777
City
Houston
State
TX
Country
United States
Zip Code
77030
Kubo, S; Rodriguez Jr, T; Roh, M S et al. (1994) Stimulation of phagocytic activity of murine Kupffer cells by tuftsin. Hepatology 19:1044-9
Nishioka, K; Wagle, J R; Rodriguez Jr, T et al. (1994) Studies of human granulocyte phagocytosis stimulation by tuftsin. J Surg Res 56:94-101
Nishioka, K; Dessens, S E; Rodriguez Jr, T (1991) Stability of sterile saline solutions of synthetic tuftsin, a naturally occurring immunomodulating peptide. Pept Res 4:230-3
Nishioka, K; Hurr, K J; Dessens, S E et al. (1991) A comparative study of [Leu1]Tuftsin and tuftsin, a natural phagocytosis-stimulating peptide. Int J Biochem 23:627-30
Nishioka, K; Wagle, J R; Minter, A M et al. (1990) Tuftsin-enhanced thymidine incorporation by murine splenic monocytes. Int J Immunopharmacol 12:905-8
Wagle, J R; Ansevin, A T; Dessens, S E et al. (1989) Specific translocation of tuftsin (Thr-Lys-Pro-Arg), a natural immunomodulating peptide, into the nuclei of human monocytes. Biochem Biophys Res Commun 159:1147-53