Although cellular proto-oncogenes are suspected of playing important roles in both normal and abnormal cell proliferation, and in the process of differentiation, the true functions of most proto-oncogenes remain hypothetical. In the proposed research, we will investigate the participation of two nuclear proto- oncogenes, c-myc and c-fos, in the transition from quiescence to renewed growth, in normal steady state growth, in abnormal proliferation in vivo, and during induced differentiation of several types of cells. We will carry out this determination by constructing recombinants that permit exogeneous manipulation, either enhancement or blockage, of the expression of these two proto-oncogenes in transfected or microinjected cells. Our preliminary studies include an example of the successful application of this strategy, demonstrating for the first time a normal cellular process for which c-fos is necessary, the transition from the quiescent state into renewed growth by fibroblasts.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Research Project (R01)
Project #
5R01CA046676-03
Application #
3190050
Study Section
Cellular Biology and Physiology Subcommittee 1 (CBY)
Project Start
1988-03-01
Project End
1991-06-30
Budget Start
1990-03-01
Budget End
1991-06-30
Support Year
3
Fiscal Year
1990
Total Cost
Indirect Cost
Name
Wistar Institute
Department
Type
DUNS #
042250712
City
Philadelphia
State
PA
Country
United States
Zip Code
19104
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