It is the objective of the proposed investigation to determine the causal role of HPV types 16 and 18, as well as other relevant types in the etiology of human cervical cancer either alone or in combination with other known or suspected risk factors. Some 10,600 women aged 20-29 years will be selected at random among residents of these age groups in the municipality of Copenhagen, Denmark. They will be invited personally to participate in the study comprising the collection of information on potential risk factors by questionnaire, and an initial gynecological screening. Cells will be collected from the portio and endocervical canal, a cervical-vaginal smear will be taken, and 10 ml of blood will be drawn. Cervical epithelial cells will be stored as well as serum at - 80 degrees C. All cervical smears will be subjected immediately to diagnosis of cervical neoplasia. The women enrolled in the study will be followed up by computerized record linkage with the Danish Central Population Register, the National Death Register, the National Danish Cancer Register, and the pathology registers. By this procedure cases of cervical neoplasia as well as women with persisting normal smears will be identified; women will be invited for a rescreen, including the collection of cervical epithelial cells for HPV hybridization three years after the initial screening. During a three year follow-up period an estimated 101 cases of severe dysplasia and carcinoma in situ will develop among women with a normal smear at the initial screening period. Five controls with a normal smear will be individually matched to these cases. For cases and controls, typespecific diagnostics of HPV in the initial scrape will be performed by Vira-Pap and Vira-Type; and possibly using PCR-technique. HSV-II antibodies in the sera will be diagnosed by ELISA-technique. Analysis will be performed as a matched case-control nested within the cohort.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Research Project (R01)
Project #
5R01CA047812-02
Application #
3191589
Study Section
Epidemiology and Disease Control Subcommittee 2 (EDC)
Project Start
1990-09-20
Project End
1995-08-31
Budget Start
1991-09-01
Budget End
1992-08-31
Support Year
2
Fiscal Year
1991
Total Cost
Indirect Cost
Name
Danish Cancer Society
Department
Type
DUNS #
City
Copenhagen
State
Country
Denmark
Zip Code
Brogaard, Kim Agerholm; Munk, Christian; Iftner, Thomas et al. (2014) Detection of oncogenic genital human papillomavirus (HPV) among HPV negative older and younger women after 7 years of follow-up. J Med Virol 86:975-82
Jensen, Kirsten E; Thomsen, Louise T; Schmiedel, Sven et al. (2014) Chlamydia trachomatis and risk of cervical intraepithelial neoplasia grade 3 or worse in women with persistent human papillomavirus infection: a cohort study. Sex Transm Infect 90:550-5
Jensen, K E; Schmiedel, S; Norrild, B et al. (2013) Parity as a cofactor for high-grade cervical disease among women with persistent human papillomavirus infection: a 13-year follow-up. Br J Cancer 108:234-9
Jensen, Kirsten Egebjerg; Schmiedel, Sven; Frederiksen, Kirsten et al. (2012) Risk for cervical intraepithelial neoplasia grade 3 or worse in relation to smoking among women with persistent human papillomavirus infection. Cancer Epidemiol Biomarkers Prev 21:1949-55
Kjær, Susanne K; Frederiksen, Kirsten; Munk, Christian et al. (2010) Long-term absolute risk of cervical intraepithelial neoplasia grade 3 or worse following human papillomavirus infection: role of persistence. J Natl Cancer Inst 102:1478-88
Nielsen, Ann; Kjaer, Susanne K; Munk, Christian et al. (2010) Persistence of high-risk human papillomavirus infection in a population-based cohort of Danish women. J Med Virol 82:616-23
Nielsen, Ann; Iftner, Thomas; Munk, Christian et al. (2009) Acquisition of high-risk human papillomavirus infection in a population-based cohort of Danish women. Sex Transm Dis 36:609-15
Nielsen, Ann; Kjaer, Susanne Kruger; Munk, Christian et al. (2008) Type-specific HPV infection and multiple HPV types: prevalence and risk factor profile in nearly 12,000 younger and older Danish women. Sex Transm Dis 35:276-82
Mueller, Luise L; Munk, Christian; Thomsen, Birthe L et al. (2007) The influence of parity and smoking in the social environment on tobacco consumption among daily smoking women in Denmark. Eur Addict Res 13:177-84
Kjaer, Susanne; Hogdall, Estrid; Frederiksen, Kirsten et al. (2006) The absolute risk of cervical abnormalities in high-risk human papillomavirus-positive, cytologically normal women over a 10-year period. Cancer Res 66:10630-6

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