The regulatory elements of the E. coli lactose operon have recently been found to function appropriately in mammalian cells. One result is an ability to tightly regulate the transcription of certain eukaryotic genes by varying the concentration of the specific allolactose analog, isoprophy beta-D thiogalactoside in the culture medium. The experiments described here ask multiple generic questions. First, can one adapt the lac repressor gene to the tight and reversible repression of expression of the SV40 neoplastic transforming region? Second, if so, is it hen feasible to reversibly modulate the transforming action of the two viral transforming products, large and small T/t antigen? Success is this endeavor could, in turn, lead to specific analyses of the quantitative aspects of the in vivo function of each and, thereby, to insights into their specific biochemical contributions to the viral transforming process.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Research Project (R01)
Project #
5R01CA049530-05
Application #
3193690
Study Section
Experimental Virology Study Section (EVR)
Project Start
1989-06-01
Project End
1994-05-31
Budget Start
1993-06-01
Budget End
1994-05-31
Support Year
5
Fiscal Year
1993
Total Cost
Indirect Cost
Name
Dana-Farber Cancer Institute
Department
Type
DUNS #
149617367
City
Boston
State
MA
Country
United States
Zip Code
02215