Antifolate Inhibition of DE Novo Purine Biosynthesis

Beardsley, G Peter

Abstract

Funding Agency

• Agency: National Institute of Health (NIH)
• Institute: National Cancer Institute (NCI)
• Type: Research Project (R01)
• Project #: 2R01CA050721-08
• Application #: 2007790
• Study Section: Experimental Therapeutics Subcommittee 1 (ET)
• Project Start: 1989-08-01
• Project End: 1999-07-30
• Budget Start: 1996-09-18
• Budget End: 1997-07-31
• Support Year: 8
• Fiscal Year: 1996

Institution

• Name: Yale University
• Department: Pediatrics
• Type: Schools of Medicine
• DUNS #: 082359691
• City: New Haven
• State: CT
• Country: United States
• Zip Code: 06520

Publications

Bulock, Karen G; Beardsley, G Peter; Anderson, Karen S (2002) The kinetic mechanism of the human bifunctional enzyme ATIC (5-amino-4-imidazolecarboxamide ribonucleotide transformylase/inosine 5'-monophosphate cyclohydrolase). A surprising lack of substrate channeling. J Biol Chem 277:22168-74

Vergis, J M; Bulock, K G; Fleming, K G et al. (2001) Human 5-aminoimidazole-4-carboxamide ribonucleotide transformylase/inosine 5'-monophosphate cyclohydrolase. A bifunctional protein requiring dimerization for transformylase activity but not for cyclohydrolase activity. J Biol Chem 276:7727-33

Reyes, V M; Greasley, S E; Stura, E A et al. (2000) Crystallization and preliminary crystallographic investigations of avian 5-aminoimidazole-4-carboxamide ribonucleotide transformylase-inosine monophosphate cyclohydrolase expressed in Escherichia coli. Acta Crystallogr D Biol Crystallogr 56:1051-4

Cinquina, C C; Grogan, E; Sun, R et al. (2000) Dihydrofolate reductase from Kaposi's sarcoma-associated herpesvirus. Virology 268:201-17

Rayl, E A; Moroson, B A; Beardsley, G P (1996) The human purH gene product, 5-aminoimidazole-4-carboxamide ribonucleotide formyltransferase/IMP cyclohydrolase. Cloning, sequencing, expression, purification, kinetic analysis, and domain mapping. J Biol Chem 271:2225-33