This application concentrates upon determining the rates and spectra of mutation arising during a single cycle of HIV and MLV vector viral replication.One objective is to develop HIV-based packaging cells needed to carry out HIV mutation rate studies. Once such HIV-based packaging cells are available, they will be utilized for HIV forward mutation rate (loss-of- function) analysis. One of the genes to be employed as a reporter is the lacZa gene. The studies to be done with MLV are an extension of already described work indicating that MLV vectors can undergo genetic rearrangement at a particularly high rate, including a high rate of transduction of sequences from infected cells. Experiments are proposed to extend MLV mutation rate analysis to a number of genes to address the sequence-dependence of mutational events.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Research Project (R01)
Project #
2R01CA050777-06A1
Application #
2093986
Study Section
AIDS and Related Research Study Section 3 (ARRC)
Project Start
1989-06-15
Project End
1998-11-30
Budget Start
1994-12-15
Budget End
1995-11-30
Support Year
6
Fiscal Year
1995
Total Cost
Indirect Cost
Name
University of Medicine & Dentistry of NJ
Department
Genetics
Type
Schools of Medicine
DUNS #
622146454
City
Piscataway
State
NJ
Country
United States
Zip Code
08854
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