This research proposal involves the development of a mammalian vector system that replicates episomally and the development of new methods for the insertion of cDNA libraries into it. Because the vector remains episomal, it can be recovered rapidly and readily. In this respect it differs from previously described vectors that integrate rapidly into the host (human) cell genome, and hence, cannot be recovered readily. This system can be used to complement defective functions of human cells where the gene is unknown and where a selection can be imparted. Part of the proposal is to continue development of the vector system. Additionally the investigators propose to identify human genes which have not previously been identified: 1) Ataxia telangiectasia, specifically complementation group D; 2) gene(s) coding for methotrexate transport, genes which in a defective form result in a common form of methotrexate resistance in cancer cells.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Research Project (R01)
Project #
1R01CA054346-01
Application #
3198868
Study Section
Genome Study Section (GNM)
Project Start
1991-05-01
Project End
1994-04-30
Budget Start
1991-05-01
Budget End
1992-04-30
Support Year
1
Fiscal Year
1991
Total Cost
Indirect Cost
Name
Stanford University
Department
Type
Schools of Arts and Sciences
DUNS #
800771545
City
Stanford
State
CA
Country
United States
Zip Code
94305