Abstract

In this proposal the investigators will focus on the following objectives: 1) to establish HPV-positive cell lines from human cervix by transfection methodologies as well as by direct culture of biopsies; 2) to investigate the regulation of the response of these cell lines to growth factors, especially TGF-a and TGF-b, using conventional monolayer culture and the collagen raft system; and 3) to colocalize TGF-b and HPV mRNA in tissue biopsies obtained from women at different stages of dysplasia including invasive cancer.The raft system will allow the investigators to test the effects of TGF-a and TGF-b on papillomavirus-containing cells under conditions which more faithfully reflect the human infection. In situ hybridization experiments will reveal whether TGF-b is expressed in abnormal cervical tissues, the patterns of expression and the relationship between TGF-b and HPV-expressing cells.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Research Project (R01)
Project #
5R01CA055892-02
Application #
3200461
Study Section
Metabolic Pathology Study Section (MEP)
Project Start
1992-04-01
Project End
1995-03-31
Budget Start
1993-04-01
Budget End
1994-03-31
Support Year
2
Fiscal Year
1993
Total Cost
Indirect Cost

  Institution

Name
Brown University
Department
Type
Schools of Medicine
DUNS #
001785542
City
Providence
State
RI
Country
United States
Zip Code
02912