Epidemiological studies provide strong evidence that diet can modify cancer incidence. Populations concuming legume-derived products exhibit significantly decreased rates of breast, colon and prostate cancer. Legume products contain an array of anticarcinogens of diversified structures. Biochemical evidence correlating their presence due to legume consumption and cancer reduction in human populations is lacking. Our long term objective is io understand the quantitative relationship between soybean consumption and cancer reduction. In this proposal, we will investigate the pharmacokinetics of soybean-derived anticarcinogens in humans. The hypothesis to be tested is that continuous consumption of soybean products is necessary to maintain constant blood and tissue levels of isoflavones. This will be tested as follows: 1. The influence of food processing on isoflavone levels will be determined in different commercial soybean products. 2. The influences of age, sex, dietary habit, and disease state on absorption, disposition, and clearance of isoflavones will be determined prior to and/or after a shot term and chronic soymilk diets. The levels of isoflavones while on an unrestricted diet, at 1 week off a soybean-derived diet, at 1-4 weeks on a soybean diet and at 1-2 weeks after withdrawal from a 4 week-soybean diet will be analyzed. These will be measured in blood, urine as well as tumorous and non-tumorous breast and colon tissues. The levels of isoflavones will be compared before and after soymilk consumption, between different age or sex groups, between Asians and non-Asians, among cancer subjects, normal vegetarians, and normal omnivores, or between tumor and non-tumorous tissues. The Greater Houston-Galveston area has a large Asian population (Chinese, Vietnamese, and Indians) accustomed to a high soybean content diet. Many individuals in this population are also vegetarians. Furthermore, large Hispanic and Black populations are also available in this area. Thus, the population composition is uniqudly suited for current pilot and future large scale dietary intervention trials. This study will provide a rational basis for future dietary studies of cancer prevention by soybean meals.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Research Project (R01)
Project #
5R01CA056273-03
Application #
2097193
Study Section
Special Emphasis Panel (SRC (48))
Project Start
1991-09-30
Project End
1995-08-31
Budget Start
1993-09-01
Budget End
1995-08-31
Support Year
3
Fiscal Year
1993
Total Cost
Indirect Cost
Name
University of Texas Medical Br Galveston
Department
Public Health & Prev Medicine
Type
Schools of Medicine
DUNS #
041367053
City
Galveston
State
TX
Country
United States
Zip Code
77555
Ramanujam, V-M S; Nayeem, Fatima; Anderson, Karl E et al. (2017) Riboflavin as an independent and accurate biomarker for adherence in a randomized double-blind and placebo-controlled clinical trial. Biomarkers 22:508-516
Ramanujam, V M Sadagopa; Anderson, Karl E; Grady, James J et al. (2011) Riboflavin as an oral tracer for monitoring compliance in clinical research. Open Biomark J 2011:1-7
Nayeem, Fatima; Nagamani, Manubai; Anderson, Karl E et al. (2009) Dietary beta-tocopherol and linoleic acid, serum insulin, and waist circumference predict circulating sex hormone-binding globulin in premenopausal women. J Nutr 139:1135-42
Huang, Yafei; Anderson, Karl E; Nagamani, Manubai et al. (2008) Dietary intake of lactose as a strong predictor for secretor status of nipple aspirate fluid in healthy premenopausal nonlactating women. Clin Cancer Res 14:1386-92
Huang, Yafei; Nagamani, Manubai; Anderson, Karl E et al. (2007) A strong association between body fat mass and protein profiles in nipple aspirate fluid of healthy premenopausal non-lactating women. Breast Cancer Res Treat 104:57-66
Lu, L J; Anderson, K E; Grady, J J et al. (2001) Effects of an isoflavone-free soy diet on ovarian hormones in premenopausal women. J Clin Endocrinol Metab 86:3045-52
Wan, X S; Lu, L J; Anderson, K E et al. (2000) Urinary excretion of Bowman-Birk inhibitor in humans after soy consumption as determined by a monoclonal antibody-based immunoassay. Cancer Epidemiol Biomarkers Prev 9:741-7
Lu, L J; Anderson, K E; Grady, J J et al. (2000) Decreased ovarian hormones during a soya diet: implications for breast cancer prevention. Cancer Res 60:4112-21
Cao, S; Hudnall, S D; Kohen, F et al. (2000) Measurement of estrogen receptors in intact cells by flow cytometry. Cytometry 41:109-14
Lu, L J; Cree, M; Josyula, S et al. (2000) Increased urinary excretion of 2-hydroxyestrone but not 16alpha-hydroxyestrone in premenopausal women during a soya diet containing isoflavones. Cancer Res 60:1299-305

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