Based on N-terminal (amino acid) and C-terminal (base) sequences, OFP appears to be a novel protein. Normally present only in the fetus and placenta, it is re-induced early in carcinogenesis, persists in the tumor and is secreted to circulation. The hypothesis is that OFP is produced in and released from pre-malignant lesions in the rat mammary gland after treatment with 7,12-dimethylbenz(a)anthracene (DMBA). The DMBA-induced model will be used to identify mammary lesions producing OFP, and to correlate rate of production and steady state blood levels as a function of time with various histopathological parameters and tumorigenic potential. In addition to tumorigenesis modulating agents (i.e., retinoid and chorionic gonadotropin) this study will apply specific monoclonal antibodies and cDNA to an ELISA assay, immunolocalization and in situ hybridization. The possible interaction of OFP with the cell membrane (receptors) will be evaluated in rat mammary cell lines. The data from this study are relevant to detection of premalignant lesions, and to monitoring chemical carcinogenesis.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Research Project (R01)
Project #
5R01CA058940-02
Application #
2099556
Study Section
Chemical Pathology Study Section (CPA)
Project Start
1993-01-01
Project End
1995-12-31
Budget Start
1994-01-01
Budget End
1994-12-31
Support Year
2
Fiscal Year
1994
Total Cost
Indirect Cost
Name
Ohio State University
Department
Biochemistry
Type
Schools of Medicine
DUNS #
098987217
City
Columbus
State
OH
Country
United States
Zip Code
43210