Molecular Biology Research will develop methods for molecular phenotyping of cancer cells by flow cytometry.
Three specific aims are proposed:
Aim one is to isolate cell populations of interest by cell sorting and determine gene amplification levels by PCR on less than 1000 cells (not the millions of cells required for standard blotting techniques).
Aims two and three are to develop two new technologies, fluorescence in-situ hybridization on suspension (FISHES (aim 2) and fluorescence in-situ polymerase chain reaction (FLIP) (aim 3). The goal of these studies is to correlate gene expression at the mRNA level by FISHES or FLIP with protein expression by immunofluorescence.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Research Project (R01)
Project #
5R01CA060200-03
Application #
2100895
Study Section
Special Emphasis Panel (SSS)
Project Start
1992-09-23
Project End
1996-03-31
Budget Start
1994-08-01
Budget End
1996-03-31
Support Year
3
Fiscal Year
1994
Total Cost
Indirect Cost
Name
Roswell Park Cancer Institute Corp
Department
Type
DUNS #
City
Buffalo
State
NY
Country
United States
Zip Code
14263