Abstract

The focus of this RO1 is on the induction, repair and mutagenicity of O- and N- oxidant generated DNA damage in cultured human epidermal keratinocytes. The hypothesis driving this research is that NO and peroxynitrite are mutagenic, and could be important contributors to oxidative DNA damage and mutation in human epidermal keratinocytes at sites of inflammation.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Research Project (R01)
Project #
5R01CA069872-05
Application #
6376215
Study Section
Chemical Pathology Study Section (CPA)
Project Start
1997-06-01
Project End
2003-03-31
Budget Start
2001-04-01
Budget End
2003-03-31
Support Year
5
Fiscal Year
2001
Total Cost
$296,650
Indirect Cost

  Institution

Name
Ohio State University
Department
Microbiology/Immun/Virology
Type
Schools of Medicine
DUNS #
098987217
City
Columbus
State
OH
Country
United States
Zip Code
43210

  Publications

Shokolenko, Inna N; Alexeyev, Mikhail F; Robertson, Fredika M et al. (2003) The expression of Exonuclease III from E. coli in mitochondria of breast cancer cells diminishes mitochondrial DNA repair capacity and cell survival after oxidative stress. DNA Repair (Amst) 2:471-82
Shokolenko, I; Oberyszyn, T M; D'Ambrosio, S M et al. (2001) Protection of human keratinocyte mtDNA by low-level nitric oxide. Nitric Oxide 5:555-60
D'Ambrosio, S M; Gibson-D'Ambrosio, R E; Brady, T et al. (2001) Mechanisms of nitric oxide-induced cytotoxicity in normal human hepatocytes. Environ Mol Mutagen 37:46-54