The Specific Aims are:
Specific Aim 1 is designed to test the hypothesis that constitutive activation of PI3 kinase and its downstream signaling pathways mediate the alter phenotypes expressed by transformed HME cells expressing varying levels of erbB-2.
Specific Aim 2 will be used to determine the mechanisms by which other oncogenes, that are amplified and/or overexpressed in human breast cancer cells, transform HME cells. This will be done by examining, in the context of the model recently developed, the ability of specific known and candidate oncogenes to induce phenotypes that are induced by erbB-2, when overexpressed to levels similar to that of breast cancer cell lines. The phenotypes to be studied include; factor independent survival/proliferation, growth in soft agar, and invasion of basement membranes.
Specific Aim 3 is designed to examine the epigenetic consequences of overexpression of erbB-2 and other putative breast cancer oncogenes in order to identify common transcriptional events mediated by these genes, and to determine their role in the acquisition of specific altered phenotypes.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Research Project (R01)
Project #
5R01CA070354-09
Application #
6744307
Study Section
Special Emphasis Panel (ZRG1-SSS-N (02))
Program Officer
Perry, Mary Ellen
Project Start
1996-07-01
Project End
2004-07-31
Budget Start
2004-05-01
Budget End
2004-07-31
Support Year
9
Fiscal Year
2004
Total Cost
$123,506
Indirect Cost
Name
University of Michigan Ann Arbor
Department
Radiation-Diagnostic/Oncology
Type
Schools of Medicine
DUNS #
073133571
City
Ann Arbor
State
MI
Country
United States
Zip Code
48109
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