This application emphasizes two complementary approaches directed at increasing the sensitivity and specificity of breast tumor detection: 1) to use optical contrast agents which occupy the vascular and extravascular volumes of the tumor to enhance the tumor contrast with respect to the normal tissue background; and 2) to exploit the remarkable sensitivity and specificity of small object detection achievable by amplitude cancellation (phased array) diffusion wave optical systems. The contrast agent of particular interest is indocyanine green, an FDA- approved dye for studying the retina, the liver, the heart and the brain and here used for optical detection of specific tumor characteristics. The method also can employ multiwavelength contrast agent detection to enhance the specificity/sensitivity and to afford detection of blood occupied vascular volume and blood deoxygenation. The work proposes extensive studies of appropriate models for the breast tumor and will focus on in vitro and in vivo models. The 9L glioma not tumor will be used because of its well-recognized and documented radioresistant characteristics. Boundary errors are minimized by the use of matching absorption (mu a) and scattering (mu s') in a human """"""""breast box."""""""" Two and three dimensional electromechanical scans are used for locating an isolated small tumor in the human breast. Detection contrast is provided by relative increases of blood volume and contrast agent accumulation in vascular and extravascular spaces of the tumor with respect to the normal tissue. Further developments will provide hemoglobin deoxygenation, fluorescent contrast agents, mitochondrial light scattering and osmotic perturbation as additional sources of tumor/host tissue contrast in studies of 20 tumor bearing human breasts per year.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Research Project (R01)
Project #
7R01CA072895-03
Application #
2895787
Study Section
Special Emphasis Panel (ZRG7-DMG (01))
Program Officer
Menkens, Anne E
Project Start
1997-06-01
Project End
2001-03-31
Budget Start
1999-04-01
Budget End
2000-03-31
Support Year
3
Fiscal Year
1999
Total Cost
Indirect Cost
Name
Medical Diagnostic Research Foundation
Department
Type
DUNS #
084403778
City
Radnor
State
PA
Country
United States
Zip Code
19087
Ranji, Mahsa; Kanemoto, Shinya; Matsubara, Muneaki et al. (2006) Fluorescence spectroscopy and imaging of myocardial apoptosis. J Biomed Opt 11:064036
Chen, Yu; Intes, Xavier; Chance, Britton (2005) Development of high-sensitivity near-infrared fluorescence imaging device for early cancer detection. Biomed Instrum Technol 39:75-85
Intes, Xavier; Chance, Britton (2005) Non-PET functional imaging techniques: optical. Radiol Clin North Am 43:221-34, xii
Nioka, Shoko; Wen, Simon; Zhang, Jun et al. (2005) Simulation study of breast tissue hemodynamics during pressure perturbation. Adv Exp Med Biol 566:17-22
Nioka, S; Chance, B (2005) NIR spectroscopic detection of breast cancer. Technol Cancer Res Treat 4:497-512
Zhang, Zhihong; Li, Hui; Liu, Qian et al. (2004) Metabolic imaging of tumors using intrinsic and extrinsic fluorescent markers. Biosens Bioelectron 20:643-50
Zhang, Zhihong; Blessington, Dana; Li, Hui et al. (2004) Redox ratio of mitochondria as an indicator for the response of photodynamic therapy. J Biomed Opt 9:772-8
Chen, Y; Zheng, G; Zhang, Z H et al. (2003) Metabolism-enhanced tumor localization by fluorescence imaging: in vivo animal studies. Opt Lett 28:2070-2
Tu, Tao; Chen, Yu; Zhang, Jun et al. (2002) Analysis on performance and optimization of frequency-domain near-infrared instruments. J Biomed Opt 7:643-9
Zhu, Q; Conant, E; Chance, B (2000) Optical imaging as an adjunct to sonograph in differentiating benign from malignant breast lesions. J Biomed Opt 5:229-36

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